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1. (WO2018045014) STIMULATED EMISSION DEPLETION NONLINEAR STRUCTURED ILLUMINATION MICROSCOPY (STED-NSIM) APPARATUS, METHODS, AND APPLICATIONS

Pub. No.:    WO/2018/045014    International Application No.:    PCT/US2017/049349
Publication Date: Fri Mar 09 00:59:59 CET 2018 International Filing Date: Thu Aug 31 01:59:59 CEST 2017
IPC: H04N 5/225
G01N 21/64
G02B 21/36
G02B 27/58
Applicants: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA
Inventors: PENG, Leilei
LI, Yu
ZHANG, Han
Title: STIMULATED EMISSION DEPLETION NONLINEAR STRUCTURED ILLUMINATION MICROSCOPY (STED-NSIM) APPARATUS, METHODS, AND APPLICATIONS
Abstract:
A superresolution STED-NSEVI apparatus having an epifluorescence architecture utilizing a 2D structured STED pattern having a N.A. less than a N.A. of the microscope objective and no surface plasmon resonance (SPR) effects. A superresolution STED-NSEVI imaging method using a fully deterministic imaging processing method, in which a pre- calibrated set of parameters are used to process all image data.