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1. (WO2018044934) METHODS AND COMPOSITIONS FOR SPINAL CORD CELLS
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/044934 International Application No.: PCT/US2017/049193
Publication Date: 08.03.2018 International Filing Date: 29.08.2017
IPC:
A61K 35/24 (2015.01) ,A61K 35/30 (2015.01) ,C12M 3/00 (2006.01) ,C12N 5/0797 (2010.01)
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35
Medicinal preparations containing material or reaction products thereof with undetermined constitution
12
Materials from mammals or birds
24
Mucus; Mucous glands; Bursa; Arthral fluid; Excreta; Spinal fluid
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35
Medicinal preparations containing material or reaction products thereof with undetermined constitution
12
Materials from mammals or birds
30
Nerves; Brain
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3
Tissue, human, animal or plant cell, or virus culture apparatus
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
079
Neural cells
0797
Stem cells; Progenitor cells
Applicants: CEDARS-SINAI MEDICAL CENTER[US/US]; 8700 Beverly Blvd. Los Angeles, California 90048, US
Inventors: VATINE, Gad; US
SANCES, Sam; US
SVENDSEN, Clive; US
SAREEN, Dhruv; US
KERL, Alexis J.; US
Agent: CHEN, Stephen, W.; US
BIRKENEDER, Erik J.; US
COSTELLIA, Jeffrey; US
EISENSTEIN, Ronald, I.; US
GARETTO, Janet, M.; US
GATZ, Robert; US
HUBER, Linda B.; US
KIM, Sean; US
LEVY, Seth D.; US
MATTAN-HUNG, Natalia; US
PROMMER, Peter J.; US
RESNICK, David S.; US
SWIATEK, Maria; US
TANG, Wayne L.; US
VAKHARIA-RAO, Hema; US
HAYES, Jennifer; US
Priority Data:
15/352,28915.11.2016US
62/380,78029.08.2016US
PCT/US2016/05772419.10.2016US
Title (EN) METHODS AND COMPOSITIONS FOR SPINAL CORD CELLS
(FR) PROCÉDÉS ET COMPOSITIONS POUR CELLULES DE MOELLE ÉPINIÈRE
Abstract:
(EN) Described here are systems and methods for deriving both spinal motor neurons and brain microvascular endothelial cells from induced pluripotent stem cells using distinct methods and combining them in a chip format. Neurons cultured alone in chip microvolume displayed increased calcium transient function and chip-specific gene expression. When seeded with endothelial cells, interaction further enhanced neural function, elicited vascular-neural interaction, niche gene expression with enhanced in vivo-like signatures arising from the chip co-cultures. Development of novel media formulations further allow for improved readout of differentiation process, by eliminating additives that otherwise confound differentiation processes and resulting phenotypes.
(FR) La présente invention concerne des systèmes et des procédés pour dériver des neurones moteurs spinaux et des cellules endothéliales microvasculaires cérébrales à partir de cellules souches pluripotentes induites au moyen de procédés distincts et combiner celles-ci dans un format de puce. Les neurones cultivés seuls dans le microvolume de puce présentent une fonction transitoire calcique et une expression génique spécifique à la puce augmentées. Lorsqu’ils sont ensemencés avec des cellules endothéliales, l’interaction augmente plus avant la fonction neuronale, entraîne une interaction vasculaire-neuronale induite, l’expression génique de niche avec des signatures de type in vivo améliorées provenant des co-cultures de puce. Le développement de nouvelles formulations de milieu permet en outre d’obtenir une lecture améliorée du processus de différenciation, par élimination d’additifs qui rendent autrement les processus de différenciation et les phénotypes résultants confus.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)