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1. (WO2018044795) COMPOSITIONS AND METHODS FOR PROGRAMMING ADULT CELLS WITH PLATELET RICH FRACTION OF BLOOD CONTAINING PLATELET-LIKE CELLS

Pub. No.:    WO/2018/044795    International Application No.:    PCT/US2017/048907
Publication Date: Fri Mar 09 00:59:59 CET 2018 International Filing Date: Tue Aug 29 01:59:59 CEST 2017
IPC: C12N 5/074
C12N 5/078
C12N 5/095
Applicants: ZHAO, Yong
Inventors: ZHAO, Yong
Title: COMPOSITIONS AND METHODS FOR PROGRAMMING ADULT CELLS WITH PLATELET RICH FRACTION OF BLOOD CONTAINING PLATELET-LIKE CELLS
Abstract:
The described invention provides a method of functionally reprogramming adult cells to an immature cell type that expresses one or more embryonic biomarkers. The reprogramming is accomplished by contacting the adult cells with a platelet rich fraction comprising platelet-like cells from umbilical cord blood or peripheral blood, and expanding the immature cell type in vitro under culture conditions to generate an insulin-producing cell population that expresses human beta-cell specific transcription factors and is functionally equivalent to human pancreatic beta-cells. Without being limited by theory, platelet-like cells and their released mitochondria display immune tolerance-associated markers that may modulate the function and differentiation of immune cells. The described invention further provides a pharmaceutical composition comprising a cell product containing a therapeutic amount of an insulin-producing cell population derived from functionally reprogrammed adult cells, wherein the insulin-producing cell population expresses human beta-cell specific transcription factors and is functionally equivalent to human pancreatic beta-cells.