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1. (WO2018044619) CONDITIONALLY ACTIVE POLYPEPTIDES AND METHODS OF GENERATING THEM
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Pub. No.: WO/2018/044619 International Application No.: PCT/US2017/047950
Publication Date: 08.03.2018 International Filing Date: 22.08.2017
IPC:
C07K 16/18 (2006.01) ,C07K 16/28 (2006.01) ,G01N 33/574 (2006.01) ,A61K 39/00 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
18
against material from animals or humans
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
18
against material from animals or humans
28
against receptors, cell surface antigens or cell surface determinants
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
574
for cancer
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39
Medicinal preparations containing antigens or antibodies
Applicants:
BIOATLA, LLC [US/US]; 11085 Torreyana Road Suite 100 San Diego, California 92121, US
Inventors:
SHORT, Jay, M.; US
CHANG, Hwai, Wen; US
FREY, Gerhard; US
Agent:
DUNLEAVY, Kevin, J.; US
Priority Data:
62/382,02331.08.2016US
Title (EN) CONDITIONALLY ACTIVE POLYPEPTIDES AND METHODS OF GENERATING THEM
(FR) POLYPEPTIDES CONDITIONNELLEMENT ACTIFS ET LEURS PROCÉDÉS DE GÉNÉRATION
Abstract:
(EN) A method of preparing a conditionally active polypeptide from a parent polypeptide, comprising steps of evolving a DNA encoding the parent polypeptide by increasing a net charge of the parent polypeptide using one or more techniques selected from increasing a total number of codons of charged amino acid residues in the DNA and decreasing a total number of codons of uncharged amino acid residues in the DNA to create mutant DNAs; expressing the mutant DNAs to obtain mutant polypeptides; and selecting the conditionally active polypeptide from the mutant polypeptides which exhibits a decrease in activity in a first assay at a first value of a condition compared to the same activity in a second assay at a second value of the same condition. The conditionally active polypeptide, pharmaceutical compositions containing same, nanoparticle and drug conjugates thereof and uses thereof are also provided.
(FR) L'invention concerne un procédé de préparation d'un polypeptide conditionnellement actif à partir d'un polypeptide parent, comprenant les étapes consistant à faire évoluer un ADN codant pour le polypeptide parent par l'augmentation d'une charge nette du polypeptide parent à l'aide d'un ou des techniques choisies parmi l'augmentation d'un nombre total de codons de résidus d'acides aminés chargés dans l'ADN et la réduction d'un nombre total de codons de résidus d'acides aminés non chargés dans l'ADN pour créer des ADN mutants; à exprimer les ADN mutants pour obtenir des polypeptides mutants; et à sélectionner le polypeptide conditionnellement actif à partir des polypeptides mutants qui présente une diminution de l'activité dans un premier dosage à une première valeur d'une condition par rapport à la même activité dans un second dosage à une seconde valeur de la même condition. L'invention concerne également le polypeptide conditionnellement actif, des compositions pharmaceutiques les contenant, des conjugués de nanoparticules et de médicament de ceux-ci et leurs utilisations.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)