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1. (WO2018043688) ANALYTE CONCENTRATION MEASURING METHOD, PARTICLE CONTAINING AGGLUTINATED FLUORESCENT MATERIAL, AND INSPECTION DEVICE

Pub. No.:    WO/2018/043688    International Application No.:    PCT/JP2017/031500
Publication Date: Fri Mar 09 00:59:59 CET 2018 International Filing Date: Fri Sep 01 01:59:59 CEST 2017
IPC: G01N 33/543
G01N 21/64
Applicants: SEKISUI CHEMICAL CO., LTD.
積水化学工業株式会社
SEKISUI MEDICAL CO., LTD.
積水メディカル株式会社
Inventors: IWAMOTO Tadashi
岩本 匡志
SUGIMOTO Satoru
杉本 理
WAKIYA Takeshi
脇屋 武司
KITAHARA Shinichiro
北原 慎一郎
IKEGAMI Maasa
池上 真亜紗
Title: ANALYTE CONCENTRATION MEASURING METHOD, PARTICLE CONTAINING AGGLUTINATED FLUORESCENT MATERIAL, AND INSPECTION DEVICE
Abstract:
This analyte concentration measuring method is provided with: a step of preparing a mixed solution by mixing a sample solution containing an analyte, with a solution containing agglutinating-fluorescent-material-containing particles that have a binding partner which binds with the analyte and that agglutinate and fluoresce when the analyte binds to the binding partner; a step of measuring the fluorescence intensity generated from the agglutinating-fluorescent-material-containing particles in the mixed solution; and a step of comparing a fluorescence intensity calibration curve for analyte concentration with the fluorescence intensity, and associating the fluorescence intensity with the analyte concentration in the mixed solution. Employing agglutinating-luminescent-material-containing particles enables measurements to be carried out with a satisfactory detection sensitivity while suppressing background fluorescence.