Search International and National Patent Collections

1. (WO2018043660) METHOD FOR MANUFACTURING NATURAL-KILLER-T (NKT)-CELL STIMULATING DENDRITIC CELL AND METHOD FOR MANUFACTURING CELL COMPOSITION CONTAINING NKT-CELL STIMULATING DENDRITIC CELL AND NKT CELL

Pub. No.:    WO/2018/043660    International Application No.:    PCT/JP2017/031419
Publication Date: Fri Mar 09 00:59:59 CET 2018 International Filing Date: Fri Sep 01 01:59:59 CEST 2017
IPC: C12N 5/0784
C12N 5/0783
C12N 5/0786
Applicants: RIKEN IMMUNE REGENERATIVE MEDICINE INC.
株式会社理研免疫再生医学
Inventors: NOGUCHI Katsuo
野口 活夫
TADAKI Toshio
只木 敏雅
Title: METHOD FOR MANUFACTURING NATURAL-KILLER-T (NKT)-CELL STIMULATING DENDRITIC CELL AND METHOD FOR MANUFACTURING CELL COMPOSITION CONTAINING NKT-CELL STIMULATING DENDRITIC CELL AND NKT CELL
Abstract:
Provided is a method for manufacturing NKT-cell stimulating dendritic cells, the method including: a step for placing mononuclear cells in a culturing vessel and allowing some of the mononuclear cells to settle on a bottom surface of the vessel by keeping the culture still; a step for removing floating cells other than the cells that have settled on the bottom surface of the culturing vessel; a step for causing monocytes among the cells that have settled on the bottom surface to differentiate into immature dendritic cells by adding a predetermined factor to the culturing vessel; and a step for inducing mature dendritic cells into NKT-cell stimulating dendritic cells by adding α-galactosylceramide to the culturing vessel.