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1. (WO2018042019) EUKARYOTIC ADULT CELLS COMPRISING A TRANSFECTION SYSTEM AND THE PRODUCTION AND USE THEREOF
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Pub. No.: WO/2018/042019 International Application No.: PCT/EP2017/072018
Publication Date: 08.03.2018 International Filing Date: 01.09.2017
IPC:
C12N 15/11 (2006.01) ,C12N 15/113 (2010.01) ,C12N 15/87 (2006.01) ,B82Y 5/00 (2011.01) ,H01F 1/00 (2006.01) ,H01F 1/03 (2006.01) ,A61K 9/51 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
113
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
87
Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
B PERFORMING OPERATIONS; TRANSPORTING
82
NANO-TECHNOLOGY
Y
SPECIFIC USES OR APPLICATIONS OF NANO-STRUCTURES; MEASUREMENT OR ANALYSIS OF NANO-STRUCTURES; MANUFACTURE  OR TREATMENT OF NANO-STRUCTURES
5
Nano-biotechnology or nano-medicine, e.g. protein engineering or drug delivery
H ELECTRICITY
01
BASIC ELECTRIC ELEMENTS
F
MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
1
Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
H ELECTRICITY
01
BASIC ELECTRIC ELEMENTS
F
MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
1
Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
01
of inorganic materials
03
characterised by their coercivity
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
9
Medicinal preparations characterised by special physical form
48
Preparations in capsules, e.g. of gelatin, of chocolate
50
Microcapsules
51
Nanocapsules
Applicants:
UNIVERSITÄT ROSTOCK [DE/DE]; Universitätsplatz 1 18055 Rostock, DE
Inventors:
STEINHOFF, Gustav; DE
DAVID, Robert; DE
MÜLLER, Paula; DE
VORONINA, Natalia; DE
HAUSBURG, Frauke; DE
Agent:
HERZOG FIESSER & PARTNER PATENTANWÄLTE PARTG MBB; Dr. Andreas Altmann Dudenstraße 46 68167 Mannheim, DE
Priority Data:
10 2016 216 657.302.09.2016DE
Title (EN) EUKARYOTIC ADULT CELLS COMPRISING A TRANSFECTION SYSTEM AND THE PRODUCTION AND USE THEREOF
(FR) CELLULES EUCARYOTES ADULTES COMPRENANT UN SYSTÈME DE TRANSFECTION ET PRODUCTION ET UTILISATION DE CES CELLULES
(DE) EUKARYOTISCHE ADULTE ZELLEN UMFASSEND EIN TRANSFEKTIONSSYSTEM UND DEREN HERSTELLUNG UND VERWENDUNG
Abstract:
(EN) The present invention relates to a eukaryotic adult cell which comprises a transfection system, said transfection system comprising (i) a cationic polymer that carries amino groups which are at least partially biotinylated, (ii) magnetic nanoparticles that have streptavidin molecules bound thereto, and (ii) at least one nucleic acid.
(FR) La présente invention concerne des cellules eucaryotes adultes comprenant un système de transfection, ce système de transfection comprenant (i) un polymère cationique présentant des groupes amino qui sont au moins partiellement biotinylés, (ii) des nanoparticules magnétiques auxquelles sont liées des molécules de streptavidine, (ii) au moins un acide nucléique.
(DE) Die vorliegende Erfindung betrifft eine eukaryotische adulte Zelle umfassend ein Transfektionssystem, wobei das Transfektionssystem (i) ein kationisches Polymer aufweisend Aminogruppen, welche zumindest teilweise biotinyliert sind, (ii) magnetische Nanopartikel mit daran gebundenen Streptavidin-Molekülen, (ii) mindestens eine Nukleinsäure, umfasst.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: German (DE)
Filing Language: German (DE)