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1. (WO2018041740) NON-COVALENT DISPLAY SYSTEM USING FIMGT/DSF

Pub. No.:    WO/2018/041740    International Application No.:    PCT/EP2017/071444
Publication Date: Fri Mar 09 00:59:59 CET 2018 International Filing Date: Sat Aug 26 01:59:59 CEST 2017
IPC: C12N 15/10
Applicants: BAYER PHARMA AKTIENGESELLSCHAFT
Inventors: MUELLER, Volker, Steffen
Title: NON-COVALENT DISPLAY SYSTEM USING FIMGT/DSF
Abstract:
This invention is related to a novel non-covalent display system for use of immobilization of proteins on the surface of cells. This system is a powerful tool for library selection and protein engineering. The system relies on the capture of secreted proteins on the cell surface by using the very stable interaction of the pair FimGT/DsF. The field also encompasses the use of this method to generate cells which produce a desired level of secreted protein or secreted protein of a particular characteristic(s). In particular, the method allows rapid isolation of high expression recombinant antibody producing cell lines, or may be applied directly to rapid isolation of antibodies with specific characteristics. This method is applicable for any cell which secretes protein.