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1. (WO2018041062) MULTI-POSITION DOUBLE-TAG CONNECTOR SET FOR DETECTING GENE MUTATION AND PREPARATION METHOD THEREFOR AND APPLICATION THEREOF
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Pub. No.: WO/2018/041062 International Application No.: PCT/CN2017/099255
Publication Date: 08.03.2018 International Filing Date: 28.08.2017
IPC:
C12Q 1/68 (2006.01) ,C12N 15/11 (2006.01) ,C12N 15/10 (2006.01) ,C40B 50/06 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
40
COMBINATORIAL TECHNOLOGY
B
COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
50
Methods of creating libraries, e.g. combinatorial synthesis
06
Biochemical methods, e.g. using enzymes or whole viable micro-organisms
Applicants: AMOY DIAGNOSTICS CO., LTD[CN/CN]; No.39 Dingshan Road, Haicang District Xiamen, Fujian 361027, CN
Inventors: JIN, Baolei; CN
LI, Xuchao; CN
LIN, Qinghua; CN
SHI, Weijie; CN
GE, Huijuan; CN
RUAN, Li; CN
Agent: SHOUCHUANG JUNHE PATENT AGENT CO., LTD. XIAMEN; Room 606, No.23-3, Wanghai Road, Xiamen Software Park Phase II, Siming District Xiamen, Fujian 361012, CN
Priority Data:
201610754636.429.08.2016CN
Title (EN) MULTI-POSITION DOUBLE-TAG CONNECTOR SET FOR DETECTING GENE MUTATION AND PREPARATION METHOD THEREFOR AND APPLICATION THEREOF
(FR) ENSEMBLE DE CONNECTEURS À DOUBLE ÉTIQUETTE MULTI-POSITIONS POUR DÉTECTION D'UNE MUTATION GÉNIQUE ET SON PROCÉDÉ DE PRÉPARATION ET SON APPLICATION
(ZH) 一种用于检测基因突变的多定位双标签接头组及其制备方法和应用
Abstract:
(EN) A multi-position double-tag connector set for detecting gene mutation and preparation method therefor and application thereof, the multi-position double-tag connector set comprising a double-tag connector A, a double-tag connector B and a double-tag connector C. The double-tag connector A, the double-tag connector B and the double-tag connector C are obtained respectively by means of synthesizing a connector primer P5 with a connector primer P7-A, a connector primer P7-B and a connector primer P7-C of 5' ends which are all modified with biotin. Using the multi-position double-tag connector set, the mutation rate of 1×10 -5 genes may be accurately detected and the sensitivity of gene mutation detection may be effectively improved. A plurality of mutation sites of a plurality of genes may be detected by one-time sequencing in combination with throughput of high-throughput sequencing.
(FR) L'invention concerne un ensemble de connecteurs à double étiquette multi-positions pour la détection d'une mutation génique et son procédé de préparation et son application, l'ensemble de connecteurs à double étiquette multi-positions comprenant un connecteur à double étiquette A, un connecteur à double étiquette B et un connecteur à double étiquette C. Le connecteur à double étiquette A, le connecteur à double étiquette B et le connecteur à double étiquette C sont respectivement obtenus par synthèse d'une amorce de connecteur P5 à l'aide d'une amorce de connecteur P7-A, d'une amorce de connecteur P7-B et d'une amorce de connecteur P7-C d'extrémités 5' qui sont toutes modifiées à l'aide de biotine. À l'aide de l'ensemble de connecteurs à double étiquette multi-positions, le taux de mutation de 1 × 10-5 gènes peut être détecté avec précision et la sensibilité de détection des mutations géniques peut être efficacement améliorée. Une pluralité de sites de mutation d'une pluralité de gènes peut être détectée par séquençage unique en combinaison avec les résultats d'un séquençage à haut débit.
(ZH) 一种用于检测基因突变的多定位双标签接头组及其制备方法和应用,该多定位双标签接头组包括双标签接头A、双标签接头B和双标签接头C,双标签接头A、双标签接头B和双标签接头C分别由接头引物P5分别与5'端均修饰有生物素的接头引物P7-A、接头引物P7-B和接头引物P7-C合成而得到。利用该多定位双标签接头组,可以准确检测1×10 -5基因突变率,有效提高基因突变检测的灵敏度,结合高通量测序的通量,一次测序可以检测多个基因的多个突变位点。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)