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1. (WO2018040962) METHOD FOR BUILDING LIBRARY AND METHOD FOR SNP TYPING

Pub. No.:    WO/2018/040962    International Application No.:    PCT/CN2017/098214
Publication Date: Fri Mar 09 00:59:59 CET 2018 International Filing Date: Tue Aug 22 01:59:59 CEST 2017
IPC: C12Q 1/68
Applicants: GUANGZHOU CONSIDERIN GENE & HEALTH TECHNOLOGY CO., LTD
广州康昕瑞基因健康科技有限公司
Inventors: SHENG, Sitong
盛司潼
HUANG, Siqiang
黄思强
WEI, Ming
卫明
Title: METHOD FOR BUILDING LIBRARY AND METHOD FOR SNP TYPING
Abstract:
Provided is a method for building a library, which comprises: PCR amplifying a sample to be sequenced to obtain an amplification product; ligating linker one onto the amplification product to obtain a ligation product containing an IIS type restriction enzyme recognition sequence and an IIS type restriction enzyme cleavage site, and linker one is a double-stranded nucleic acid molecule, and the distance between the IIS type restriction enzyme cleavage site and the SNP site to be detected is 0 to 5 bases; using the IIS type restriction enzyme to cleave the ligation product to obtain a first nucleic acid fragment containing the SNP site to be detected and linker one, and forming a first end by enzyme cleaving the first nucleic acid fragment; ligating linker two at the first end of the first nucleic acid fragment to obtain a library molecule, and linker two is a double-stranded nucleic acid molecule containing a binding site of the sequencing primers. Also provided are a SNP typing method and a kit for detecting a SNP site mutation. The method shortens the detection time for sites, improves the accuracy of the detection, and unifies the sequencing primers for detecting multiple sites in the same system.