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1. (WO2018040961) METHOD FOR BUILDING LIBRARY AND METHOD FOR SNP TYPING

Pub. No.:    WO/2018/040961    International Application No.:    PCT/CN2017/098213
Publication Date: Fri Mar 09 00:59:59 CET 2018 International Filing Date: Tue Aug 22 01:59:59 CEST 2017
IPC: C12Q 1/68
Applicants: GUANGZHOU CONSIDERIN GENE & HEALTH TECHNOLOGY CO., LTD
广州康昕瑞基因健康科技有限公司
Inventors: SHENG, Sitong
盛司潼
HUANG, Siqiang
黄思强
WEI, Ming
卫明
Title: METHOD FOR BUILDING LIBRARY AND METHOD FOR SNP TYPING
Abstract:
Provided is a method for building a library, which comprises: A. performing PCR amplification on a sample to be sequenced containing a SNP site to be detected using a specific amplification primer set to obtain an amplification product; at least one primer in the primer set contains an IIS type restriction enzyme recognition sequence, and the amplification product contains an IIS type restriction enzyme cleavage site, and the distance between the IIS type restriction enzyme cleavage site and the SNP site to be detected is 0 to 5 bases; B. using the IIS type restriction enzyme to cleave the amplification product to obtain a first nucleic acid fragment containing the SNP site to be detected, and forming a first end by enzyme cleaving the first nucleic acid fragment; and C. ligating the first nucleic acid fragment with a sequencing linker at the first end under a ligase to obtain a library molecule. Also provided is a method for SNP typing. The method shortens the detection time for sites, improves the accuracy of the detection, and unifies the sequencing primers for detecting multiple sites in the same system.