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1. (WO2018040960) GENE SEQUENCING REACTION CHAMBER

Pub. No.:    WO/2018/040960    International Application No.:    PCT/CN2017/098211
Publication Date: Fri Mar 09 00:59:59 CET 2018 International Filing Date: Tue Aug 22 01:59:59 CEST 2017
IPC: C12M 1/00
Applicants: GUANGZHOU CONSIDERIN GENE & HEALTH TECHNOLOGY CO., LTD
广州康昕瑞基因健康科技有限公司
Inventors: SHENG, Sitong
盛司潼
ZHU, Jie
祝捷
Title: GENE SEQUENCING REACTION CHAMBER
Abstract:
Disclosed is a gene sequencing reaction chamber, comprising a first substrate, a second substrate and a reagent backflow substrate, wherein a front face of the second substrate is provided with a first groove, a bottom face of the first groove is provided with a first reagent backflow opening and a second reagent backflow opening; the front face of the second substrate is also provided with a reagent inlet and a reagent outlet; the first substrate is adhered to the front face of the second substrate, and the first substrate and an inner wall of the first groove enclose a reaction channel; a first reagent backflow zone and a second reagent backflow zone are formed between the reagent backflow substrate and the second substrate, wherein the first reagent backflow zone is used for connecting the reagent inlet with the first reagent backflow opening, and the second reagent backflow zone is used for connecting the second reagent backflow opening with the reagent outlet. It is unnecessary to drill a hole in the first substrate of the gene sequencing reaction chamber, so the situation where the first substrate is prone to breakage due to drilling can be avoided, improving the production yield of the first substrate, and reducing costs.