Search International and National Patent Collections

1. (WO2018039139) HYDROGEL PLATFORM FOR AQUEOUS TWO-PHASE CONCENTRATION OF A TARGET TO ENHANCE ITS DETECTION

Pub. No.:    WO/2018/039139    International Application No.:    PCT/US2017/047849
Publication Date: Fri Mar 02 00:59:59 CET 2018 International Filing Date: Tue Aug 22 01:59:59 CEST 2017
IPC: G01N 33/53
G01N 33/543
G01N 33/558
G01N 33/559
Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventors: KAMEI, Daniel Takashi
WU, Benjamin Ming
PEREIRA, David Yuan
WU, Chloe Michelle
YEE, Matthew Foosing
KEEFE, Joshua Anthony
PEARCE, Christina Caroline
DAILAMY, Amir Adam
WONG, Vincent K.
LE, Nguyen Khoi
Title: HYDROGEL PLATFORM FOR AQUEOUS TWO-PHASE CONCENTRATION OF A TARGET TO ENHANCE ITS DETECTION
Abstract:
It was discovered that hydrogel scaffolds can be used to induce phase separation as aqueous two-phase systems (ATPSs) pass through and/or rehydrate the scaffolds, allowing for concentration of target analyte(s) (e.g., biomolecule(s)) into a particular phase of the ATPS or into a leading front. Accordingly, in various embodiments methods and devices are provided that utilize aqueous two-phase systems and hydrogel scaffolds to improve the sensitivity of assays (e.g., of point-of-care assays) without sacrificing cost or ease of use.