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1. (WO2018038243) METHOD FOR PRODUCING ANTIBODY FUSION PROTEIN

Pub. No.:    WO/2018/038243    International Application No.:    PCT/JP2017/030470
Publication Date: Fri Mar 02 00:59:59 CET 2018 International Filing Date: Sat Aug 26 01:59:59 CEST 2017
IPC: C07K 19/00
A61K 39/395
C07K 16/28
C12N 9/16
C12N 5/10
C12N 15/09
Applicants: JCR PHARMACEUTICALS CO., LTD.
JCRファーマ株式会社
Inventors: KOSHIMURA, Yuri
越村 友理
SONODA, Hiroyuki
薗田 啓之
MATEV, Miroslav
マテブ ミロスラブ
KAKIMOTO Shinji
柿本 真司
FUKUI, Tsuyoshi
福井 剛
HATANO, Yukichi
秦野 勇吉
Title: METHOD FOR PRODUCING ANTIBODY FUSION PROTEIN
Abstract:
[Problem] To provide a method for producing a fusion protein obtained by fusing an antibody with a lysosomal enzyme. [Solution] A method for producing a fusion protein obtained by fusing an antibody with a human lysosomal enzyme, wherein the production method includes (a) a step for culturing mammalian cells that produce the fusion protein in serum-free medium to cause the fusion protein to be secreted in the culture broth, (b) a step for recovering the culture supernatant by removing the mammalian cells from the culture broth, and (c) a step for purifying the fusion protein from the culture supernatant using column chromatography in which a material to which is bonded a substance having an affinity for the fusion protein is used as the stationary phase, column chromatography in which a material having an affinity for phosphate groups is used as the stationary phase, and size-exclusion column chromatography.