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1. (WO2018038232) METHOD FOR PRODUCING AMPLIFICATION PRODUCT OF TARGET NUCLEIC ACID, AND USE OF SAID METHOD

Pub. No.:    WO/2018/038232    International Application No.:    PCT/JP2017/030425
Publication Date: Fri Mar 02 00:59:59 CET 2018 International Filing Date: Fri Aug 25 01:59:59 CEST 2017
IPC: C12N 15/09
C12Q 1/68
Applicants: TOHOKU UNIVERSITY
国立大学法人東北大学
Inventors: KAWASE Mitsuo
川瀬 三雄
Title: METHOD FOR PRODUCING AMPLIFICATION PRODUCT OF TARGET NUCLEIC ACID, AND USE OF SAID METHOD
Abstract:
Provided is a method for producing an amplification product of a target nucleic acid on the basis of a novel principle. This method for producing an amplification product of a target nucleic acid is provided with an amplification step for carrying out a nucleic acid amplification reaction on a test sample that may contain the target nucleic acid using a first primer, a second primer, and a nucleic acid polymerase having strand displacement activity. The amplification step is a step for using two or more oligonucleotides capable of promoting dissociation of the double strand of the target nucleic acid.