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1. (WO2018038115) COMPOSITION FOR CRYOPRESERVATION OF BOVINE REPRODUCTIVE CELLS AND CRYOPRESERVATION METHOD THEREFOR

Pub. No.:    WO/2018/038115    International Application No.:    PCT/JP2017/029979
Publication Date: Fri Mar 02 00:59:59 CET 2018 International Filing Date: Wed Aug 23 01:59:59 CEST 2017
IPC: A01N 1/02
C12N 5/073
C12N 5/076
Applicants: BIOVERDE INC.
株式会社バイオベルデ
Inventors: KUBOTA, Chikara
窪田 力
ANDO, Takaaki
安藤 貴朗
HYON, Suong-Hyu
玄 丞烋
GEN, Yuki
玄 優基
IMAMURA, Shuji
今村 修二
GESSEI, Satoru
月精 悟
Title: COMPOSITION FOR CRYOPRESERVATION OF BOVINE REPRODUCTIVE CELLS AND CRYOPRESERVATION METHOD THEREFOR
Abstract:
Provided are a liquid for cryopreservation of bovine reproductive cells such as bovine sperms and a cryopreservation method therefor. Use is made of a cryopreservation liquid wherein 0.3-0.9 w/w% of an amphoteric polyelectrolyte (an antifreeze polyamino acid), said amphoteric polyelectrolyte being prepared by, relative to 50-99 mol% of amino groups in ε-poly-L-lysine having a number-average molecular weight of 1,000-20,000, reacting succinic anhydride and thus introducing an appropriate amount of carboxyl groups thereinto, and 2-4 w/w% of glycerol are dissolved in a physiological solution. A preferred embodiment of the cryopreservation method comprises: a first dilution step for diluting bovine semen 2.5-10 fold with a physiological solution and maintaining at 2-8oC; and a second dilution step for adding dropwise a physiological solution containing the amphoteric polyelectrolyte (antifreeze polyamino acid) and glycerol to the suspension obtained in the first dilution step while maintaining at 2-8oC so as to further dilute the bovine semen 5-20 fold.