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1. (WO2018038115) COMPOSITION FOR CRYOPRESERVATION OF BOVINE REPRODUCTIVE CELLS AND CRYOPRESERVATION METHOD THEREFOR
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/038115 International Application No.: PCT/JP2017/029979
Publication Date: 01.03.2018 International Filing Date: 22.08.2017
Chapter 2 Demand Filed: 21.06.2018
IPC:
A01N 1/02 (2006.01) ,C12N 5/073 (2010.01) ,C12N 5/076 (2010.01)
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
N
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
1
Preservation of bodies of humans or animals, or parts thereof
02
Preservation of living parts
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
073
Embryonic cells or tissues; Foetal cells or tissues
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
076
Sperm cells; Spermatogonia
Applicants: BIOVERDE INC.[JP/JP]; 43-1, Minamimatsunoki-cho, Higashikujo, Minami-ku, Kyoto-shi, Kyoto 6018023, JP
Inventors: KUBOTA, Chikara; JP
ANDO, Takaaki; JP
HYON, Suong-Hyu; JP
GEN, Yuki; JP
IMAMURA, Shuji; JP
GESSEI, Satoru; JP
Agent: SHINJYU GLOBAL IP; South Forest Bldg., 1-4-19, Minamimori-machi, Kita-ku, Osaka-shi, Osaka 5300054, JP
Priority Data:
2016-16221222.08.2016JP
Title (EN) COMPOSITION FOR CRYOPRESERVATION OF BOVINE REPRODUCTIVE CELLS AND CRYOPRESERVATION METHOD THEREFOR
(FR) COMPOSITION POUR LA CRYOCONSERVATION DE CELLULES REPRODUCTRICES BOVINES ET PROCÉDÉ DE CRYOCONSERVATION ASSOCIÉ
(JA) ウシ生殖細胞の凍結保存用組成物及び凍結保存方法
Abstract:
(EN) Provided are a liquid for cryopreservation of bovine reproductive cells such as bovine sperms and a cryopreservation method therefor. Use is made of a cryopreservation liquid wherein 0.3-0.9 w/w% of an amphoteric polyelectrolyte (an antifreeze polyamino acid), said amphoteric polyelectrolyte being prepared by, relative to 50-99 mol% of amino groups in ε-poly-L-lysine having a number-average molecular weight of 1,000-20,000, reacting succinic anhydride and thus introducing an appropriate amount of carboxyl groups thereinto, and 2-4 w/w% of glycerol are dissolved in a physiological solution. A preferred embodiment of the cryopreservation method comprises: a first dilution step for diluting bovine semen 2.5-10 fold with a physiological solution and maintaining at 2-8oC; and a second dilution step for adding dropwise a physiological solution containing the amphoteric polyelectrolyte (antifreeze polyamino acid) and glycerol to the suspension obtained in the first dilution step while maintaining at 2-8oC so as to further dilute the bovine semen 5-20 fold.
(FR) L'invention concerne un liquide pour la cryoconservation de cellules reproductrices bovines telles que des spermatozoïdes bovins et un procédé de cryoconservation associé. On utilise un liquide de cryoconservation dans lequel 0,3 à 0,9 % en poids d'un polyélectrolyte amphotère (un poly-acide aminé antigel), ledit polyélectrolyte amphotère étant préparé par, par rapport à 50 à 99 % en moles de groupes amino dans l'ε-poly-L-lysine ayant un poids moléculaire moyen en nombre de 1 000 à 20 000, réaction d'anhydride succinique en introduisant ainsi une quantité appropriée de groupes carboxyle dedans, et de 2 à 4 % en poids de glycérol sont dissous dans une solution physiologique. Un mode de réalisation préféré du procédé de cryoconservation comprend : une première étape de dilution pour diluer le sperme bovin 2,5 à 10 fois avec une solution physiologique et son maintien à 2-8 oC; et une seconde étape de dilution pour ajouter goutte à goutte une solution physiologique contenant le polyélectrolyte amphotère (poly(acide aminé) antigel) et du glycérol à la suspension obtenue dans la première étape de dilution tout en la maintenant à 2-8 oC de façon à diluer encore le sperme bovin 5 à 20 fois.
(JA) ウシ精子などのウシ生殖細胞の凍結保存液及び凍結保存方法を提供する。数平均分子量1,000~20,000のε-ポリ-L-リジンのアミノ基の50~99モル%について、無水コハク酸を反応させてカルボキシル基を適当量導入した両性高分子電解質(不凍ポリアミノ酸)0.3~0.9w/w%と、グリセリン2~4w/w%とを、生理的溶液に溶解させた凍結保存液を用いる。凍結保存方法の好ましい実施形態では、ウシの精液を、生理的溶液により2.5~10倍に希釈し、2~8℃で保持する1次希釈工程と、2~8℃で保持しつつ、1次希釈工程により得られた懸濁液に、両性高分子電解質(不凍ポリアミノ酸)とグリセリンとを添加した生理的溶液を点滴により添加することで、さらに希釈することにより、ウシの精液が5~20倍に希釈されるようにする2次希釈工程とを含む。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)