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1. (WO2018038088) NUCLEIC ACID AMPLIFICATION AND DETECTION METHOD, AND SOLUTION FOR NUCLEIC ACID AMPLIFICATION AND DETECTION
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Pub. No.: WO/2018/038088 International Application No.: PCT/JP2017/029891
Publication Date: 01.03.2018 International Filing Date: 22.08.2017
IPC:
C12Q 1/68 (2006.01) ,C12N 15/09 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
Applicants:
東洋製罐グループホールディングス株式会社 TOYO SEIKAN GROUP HOLDINGS, LTD. [JP/JP]; 東京都品川区東五反田2丁目18番1号 18-1, Higashi-Gotanda 2-chome, Shinagawa-ku, Tokyo 1418627, JP
Inventors:
道志 弘輝 DOSHI, Hiroki; JP
古川 聡史 FURUKAWA, Satoshi; JP
一色 淳憲 ISSHIKI, Atsunori; JP
Agent:
特許業務法人平和国際特許事務所 HEIWA INTERNATIONAL PATENT OFFICE; 東京都千代田区神田須田町一丁目26番地 芝信神田ビル3階 Shibashin Kanda Bldg. 3rd Floor, 26, Kanda Suda-cho 1-chome, Chiyoda-ku, Tokyo 1010041, JP
Priority Data:
2016-16327424.08.2016JP
Title (EN) NUCLEIC ACID AMPLIFICATION AND DETECTION METHOD, AND SOLUTION FOR NUCLEIC ACID AMPLIFICATION AND DETECTION
(FR) PROCÉDÉ D'AMPLIFICATION ET DE DÉTECTION D'ACIDE NUCLÉIQUE, ET SOLUTION D'AMPLIFICATION ET DE DÉTECTION D'ACIDE NUCLÉIQUE
(JA) 核酸増幅及び検出方法、並びに核酸増幅及び検出用溶液
Abstract:
(EN) A nucleic acid amplification and detection method, said method comprising amplifying a target region in a nucleic acid contained in a sample by a nucleic acid amplification reaction and then detecting the amplification product of the nucleic acid through hybridization in which the nucleic acid amplification product is bound to a nucleic acid having a base sequence complementary thereto, wherein: a metal salt and/or a water-soluble polymer are added to a reaction solution for nucleic acid amplification and the nucleic acid amplification reaction is performed using the reaction solution; and the reaction solution containing the nucleic acid amplification product obtained by the nucleic acid amplification reaction is used as such in performing the hybridization.
(FR) L'invention concerne un procédé d'amplification et de détection d'acide nucléique, ledit procédé comprenant l'amplification d'une région cible dans un acide nucléique contenu dans un échantillon par une réaction d'amplification d'acide nucléique et puis la détection du produit d'amplification de l'acide nucléique par hybridation, le produit d'amplification de l'acide nucléique étant lié à un acide nucléique ayant une séquence de bases complémentaire de celui-ci; un sel métallique et/ou un polymère soluble dans l'eau étant ajoutés à une solution de réaction pour l'amplification d'acide nucléique et la réaction d'amplification d'acide nucléique étant effectuée à l'aide de la solution de réaction; et la solution de réaction contenant le produit d'amplification d'acide nucléique obtenu par la réaction d'amplification d'acide nucléique étant utilisée en tant que telle dans la réalisation de l'hybridation.
(JA) 試料中に含まれる核酸における標的領域を核酸増幅反応により増幅させ、得られた核酸増幅産物を、前記核酸増幅産物と相補的な塩基配列を有する核酸と結合させるハイブリダイゼーションを行うことによって検出する核酸増幅及び検出方法であって、核酸増幅用の反応液に金属塩及び/又は水溶性高分子を含有させて、前記反応液を用いて核酸増幅反応を行い、核酸増幅反応により得られた核酸増幅産物を含む前記反応液をそのまま用いてハイブリダイゼーションを行う。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)