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1. (WO2018037097) METHOD FOR PREPARING RICH CULTURE MEDIA FOR LABELLING AMINO ACIDS INCORPORATED INTO PROTEINS

Pub. No.:    WO/2018/037097    International Application No.:    PCT/EP2017/071380
Publication Date: Fri Mar 02 00:59:59 CET 2018 International Filing Date: Fri Aug 25 01:59:59 CEST 2017
IPC: C12P 21/00
C07K 1/13
C07B 59/00
Applicants: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
UNIVERSITE GRENOBLE ALPES
Inventors: GANS, Pierre
AWAD, Rida
BOISBOUVIER, Jérôme
HAMELIN, Olivier
KERFAH, Rime
Title: METHOD FOR PREPARING RICH CULTURE MEDIA FOR LABELLING AMINO ACIDS INCORPORATED INTO PROTEINS
Abstract:
The present invention concerns a method for preparing a microorganism hydrolysate of which at least one amino acid and/or one amino acid precursor is labelled, comprising a step of obtaining a microorganism hydrolysate in solution in which all the amino acids are in free form, and a step of replacing, in this hydrolysate, at least one amino acid and the keto acid of same, if it is present, with the same amino acid but in a labelled form or with the labelled precursor of same. The present invention also concerns enzyme mixtures for implementing this method.