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|1. (WO2018037097) METHOD FOR PREPARING RICH CULTURE MEDIA FOR LABELLING AMINO ACIDS INCORPORATED INTO PROTEINS|
|Applicants:||COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
UNIVERSITE GRENOBLE ALPES
|Title:||METHOD FOR PREPARING RICH CULTURE MEDIA FOR LABELLING AMINO ACIDS INCORPORATED INTO PROTEINS|
The present invention concerns a method for preparing a microorganism hydrolysate of which at least one amino acid and/or one amino acid precursor is labelled, comprising a step of obtaining a microorganism hydrolysate in solution in which all the amino acids are in free form, and a step of replacing, in this hydrolysate, at least one amino acid and the keto acid of same, if it is present, with the same amino acid but in a labelled form or with the labelled precursor of same. The present invention also concerns enzyme mixtures for implementing this method.