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1. WO2018034984 - TRANSGENE AND MUTATIONAL CONTROL OF SEXUALITY IN MAIZE AND RELATED GRASSES

Publication Number WO/2018/034984
Publication Date 22.02.2018
International Application No. PCT/US2017/046509
International Filing Date 11.08.2017
IPC
A01D 45/00 2006.1
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
DHARVESTING; MOWING
45Harvesting of standing crops
A01D 45/02 2006.1
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
DHARVESTING; MOWING
45Harvesting of standing crops
02of maize
A01D 45/04 2006.1
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
DHARVESTING; MOWING
45Harvesting of standing crops
04of rice
CPC
C12N 15/8212
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
821Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
8212Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
C12N 15/8213
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
8213Targeted insertion of genes into the plant genome by homologous recombination
C12N 15/8216
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
C12N 15/8218
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
C12N 15/8287
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8261with agronomic (input) traits, e.g. crop yield
8287for fertility modification, e.g. apomixis
C12N 15/8289
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8261with agronomic (input) traits, e.g. crop yield
8287for fertility modification, e.g. apomixis
8289Male sterility
Applicants
  • YALE UNIVERSITY [US]/[US]
  • RHODE ISLAND COUNCIL ON POSTSECONDARY EDUCATION [US]/[US]
Inventors
  • DELLAPORTA, Stephen L.
  • HAYWARD, Andrew
  • MORENO, Maria
  • KAUSCH, Albert
  • MOTTINGER, John
Agents
  • DOYLE, Kathryn
  • LANDRY, Brian R.
  • SILVA, Domingos J.
  • SIMPSON, Mark D.
  • MANAS, Michael
  • DRISCOLL, Stephen J.
  • EDDE, Chantal
  • CROTTY, Justin
  • PLEHN-DUJOWICH, Debora
  • YOUNG, Jacque
Priority Data
62/377,08819.08.2016US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) TRANSGENE AND MUTATIONAL CONTROL OF SEXUALITY IN MAIZE AND RELATED GRASSES
(FR) TRANSGÈNE ET CONTRÔLE MUTATIONNEL DE LA SEXUALITÉ DU MAÏS ET DE GRAMINÉES CONNEXES
Abstract
(EN) The present invention pertains to genetically modified plants, particularly maize, sorghum and rice, with an all pistillate or all staminate phenotype and methods of the same. The survival of functional pistils in maize requires the action of the sk1 gene. SK1 encodes a glycosyltransferase (GT) that protects pistils from tasselseed-mediated cell death. sk1-dependent pistil protection at a developing floret gives rise to stamen arrest at the same floret, and so determines the pistillate floral fate. This is the first single gain-of-function gene known to control sexuality. The present invention further provides a direct strategy to extend hybrid technologies to related cereals such as sorghum and rice. Tasselseed and silkless genes represent major sex determination genes in maize, a pathway that permits the efficient production of hybrid seed and the associated benefits of heterosis - increased yield, resistance to pathogens, etc. Except for maize, current hybrid systems in cereals are fraught with genetic and environmental limitations. Genotype-independent hybrid cereal technology could potentially increase crop yields as much as 20-40% without placing additional land under agricultural production. This has profound implications for food security and the environmental impact of agriculture in some of the poorest regions of the world.
(FR) La présente invention concerne des plantes génétiquement modifiées, en particulier le maïs, le sorgho et le riz, ayant un phénotype tout pistillé ou tout staminé ainsi que des procédés associés. La survie de pistils fonctionnels chez le maïs nécessite l'action du gène sk1. Le SK1 code pour une glycosyltransférase (GT) qui protège les pistils de la mort cellulaire induite par tasselseed. La protection des pistils dépendante de sk1 au niveau d'un fleuron en cours de développement donne lieu à un arrêt de l'étamine au niveau du même fleuron, et détermine ainsi le devenir floral pistillé. Il s'agit du premier gène à gain de fonction unique connu pour réguler la sexualité. La présente invention concerne en outre une stratégie directe permettant d'étendre des technologies d'hybridation à des céréales connexes telles que le sorgho et le riz. Les gènes tasselseed et silkless représentent des gènes majeurs de la détermination du sexe chez le maïs, une voie qui permet la production efficace de graines hybrides et les avantages associés d'un rendement accru par hétérosis, d'une résistance aux agents pathogènes, etc. À l'exception du maïs, les systèmes hybrides actuels chez les céréales sont truffés d'écueils avec des limitations génétiques et environnementales. La technologie de céréale hybride indépendante du génotype pourrait potentiellement augmenter les rendements des cultures jusqu'à 20 à 40 % sans placer de terre supplémentaire sous production agricole. Ceci a des implications considérables pour la sécurité des aliments et l'impact environnemental de l'agriculture dans certaines des régions les plus pauvres du monde.
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