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1. (WO2018034298) GENETICALLY-MODIFIED TRYPANOSOMA CRUZI HAVING CALCIUM INDICATOR, AND APPLICATION OF SAID TRYPANOSOMA CRUZI FOR SCREENING OF ANTI-TRYPANOSOME AGENT
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Pub. No.:    WO/2018/034298    International Application No.:    PCT/JP2017/029418
Publication Date: 22.02.2018 International Filing Date: 16.08.2017
IPC:
C12N 1/11 (2006.01), C12N 15/09 (2006.01), C12Q 1/02 (2006.01)
Applicants: RIKEN [JP/JP]; 2-1, Hirosawa, Wako-shi, Saitama 3510198 (JP)
Inventors: MIKOSHIBA Katsuhiko; (JP).
NARA Takeshi; (JP).
HASHIMOTO Muneaki; (JP)
Agent: OHNO Seiji; (JP).
MORITA Yutaka; (JP)
Priority Data:
62/375,622 16.08.2016 US
Title (EN) GENETICALLY-MODIFIED TRYPANOSOMA CRUZI HAVING CALCIUM INDICATOR, AND APPLICATION OF SAID TRYPANOSOMA CRUZI FOR SCREENING OF ANTI-TRYPANOSOME AGENT
(FR) TRYPANOSOMA CRUZI GÉNÉTIQUEMENT MODIFIÉE AYANT UN INDICATEUR DE CALCIUM, ET APPLICATION DUDIT TRYPANOSOMA CRUZI POUR LE CRIBLAGE D'UN AGENT ANTI-TRYPANOSOME
(JA) カルシウムインジケーターを有する遺伝子改変トリパノソーマ・クルージおよび抗トリパノソーマ薬のスクリーニングのためのその応用
Abstract: front page image
(EN)The present invention provides a genetically-modified Trypanosoma cruzi having a calcium indicator, and an application of said Trypanosoma cruzi for screening of an anti-trypanosome agent. More specifically, the present invention provides: a genetically-modified Trypanosoma cruzi having, in the body thereof, an intracellular calcium indicator that includes a fused protein of a mammal-derived calmodulin, a myosin light chain kinase-derived peptide (M13 peptide), and a marker protein; and a screening method of an anti-trypanosome agent in which said Trypanosoma cruzi is used.
(FR)La présente invention concerne un Trypanosoma cruzi génétiquement modifié ayant un indicateur de calcium, et une application dudit Trypanosoma cruzi pour le criblage d'un agent anti-trypanosome. Plus spécifiquement, la présente invention concerne : un Trypanosoma cruzi génétiquement modifié ayant, dans son corps, un indicateur de calcium intracellulaire qui comprend une protéine fusionnée d'une calmoduline dérivée d'un mammifère, un peptide dérivé de kinase à chaîne légère de myosine (peptide M13), et une protéine marqueur. L'invention concerne en outre un procédé de criblage d'un agent anti-trypanosome dans lequel ledit Trypanosoma cruzi est utilisé.
(JA)本発明は、カルシウムインジケーターを有する遺伝子改変トリパノソーマ・クルージおよび抗トリパノソーマ薬のスクリーニングのためのその応用を提供する。具体的には、本発明によれば、哺乳動物由来カルモジュリン、ミオシン軽鎖キナーゼ由来ペプチド(M13ペプチド)およびマーカータンパク質との融合タンパク質を含む細胞内カルシウムインジケーターを体内に有する遺伝子改変トリパノソーマ・クルージおよびこれを用いた抗トリパノソーマ薬のスクリーニング方法が提供される。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)