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1. (WO2018031686) A DEVICE FOR MASSIVELY PARALLEL HIGH THROUGHPUT SINGLE CELL ELECTROPORATION AND USES THEREOF

Pub. No.:    WO/2018/031686    International Application No.:    PCT/US2017/046152
Publication Date: Fri Feb 16 00:59:59 CET 2018 International Filing Date: Thu Aug 10 01:59:59 CEST 2017
IPC: A61N 1/32
A61N 1/30
B03C 5/00
B03C 5/02
Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventors: SANTRA, Tuhin, Subhra
TEITELL, Michael, A.
CHIOU, Pei-Yu, E.
Title: A DEVICE FOR MASSIVELY PARALLEL HIGH THROUGHPUT SINGLE CELL ELECTROPORATION AND USES THEREOF
Abstract:
In various embodiments a Massively parallel Single-cell Electroporation Platform (MSEP) for low voltage, high efficiency delivery of extracellular materials into mammalian cells at an ultrahigh throughput of 10 million cells/min on a 1 cm2 chip is provided. In certain embodiments MSEP is realized by a 3D silicon-based device with, e.g., 5,000 short vertical microfluidic channels in parallel. Single cells flowing through these channels are geometrically confined to regions with intense and localized electric fields where cells are electroporated. High efficiency delivery of calcium dyes, large-sized dextran proteins, and plasmids into mammalian cells to establish a range of sizes and compositions have been successfully accomplished with MSEP.