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1. (WO2018028403) METHOD FOR PREPARING Β-CAROTENE CRYSTAL

Pub. No.:    WO/2018/028403    International Application No.:    PCT/CN2017/093523
Publication Date: Fri Feb 16 00:59:59 CET 2018 International Filing Date: Thu Jul 20 01:59:59 CEST 2017
IPC: C12P 23/00
C07C 403/00
Applicants: CABIO BIOTECH (WUHAN) CO., LTD.
嘉必优生物技术(武汉)股份有限公司
Inventors: WANG, Zhiming
汪志明
LI, Xiangyu
李翔宇
LU, Shuhuan
陆姝欢
TIAN, Yong
田勇
ZHOU, Qiang
周强
YI, Dewei
易德伟
Title: METHOD FOR PREPARING Β-CAROTENE CRYSTAL
Abstract:
Provided is a method for preparing a β-carotene crystal, comprising: (1) inoculating micro-organisms and fermenting with same; (2) performing solid-liquid separation on the fermentation broth to obtain wet thalli; (3) drying the wet thalli to obtain dried thalli; (4) extracting the dried thalli with an organic solvent; (5) performing solid-liquid separation to obtain an extraction liquid; (6) evaporating and crystallizing the extraction liquid to obtain a crystallization mother liquor; (7) performing solid-liquid separation on the crystallization mother liquor to obtain a crude wet crystal; and (8) washing the crude wet crystal with an organic solvent, and drying same under vacuum to obtain a β-carotene crystal, wherein an antioxidant is added in one or more steps of steps (3), (4) and (6).