Search International and National Patent Collections
|1. (WO2018028403) METHOD FOR PREPARING Β-CAROTENE CRYSTAL|
|Applicants:||CABIO BIOTECH (WUHAN) CO., LTD.
|Title:||METHOD FOR PREPARING Β-CAROTENE CRYSTAL|
Provided is a method for preparing a β-carotene crystal, comprising: (1) inoculating micro-organisms and fermenting with same; (2) performing solid-liquid separation on the fermentation broth to obtain wet thalli; (3) drying the wet thalli to obtain dried thalli; (4) extracting the dried thalli with an organic solvent; (5) performing solid-liquid separation to obtain an extraction liquid; (6) evaporating and crystallizing the extraction liquid to obtain a crystallization mother liquor; (7) performing solid-liquid separation on the crystallization mother liquor to obtain a crude wet crystal; and (8) washing the crude wet crystal with an organic solvent, and drying same under vacuum to obtain a β-carotene crystal, wherein an antioxidant is added in one or more steps of steps (3), (4) and (6).