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1. (WO2018027290) HETEROLOGOUS EXPRESSION CASSETTE, DNA CONSTRUCT AND VACCINE COMPOSITION FOR IMMUNIZING AGAINST FLAVIVIRUS AND/OR OTHER PATHOGENS

Pub. No.:    WO/2018/027290    International Application No.:    PCT/BR2017/050221
Publication Date: Fri Feb 16 00:59:59 CET 2018 International Filing Date: Sat Aug 05 01:59:59 CEST 2017
IPC: C12N 15/86
C12N 15/40
C12N 15/66
A61K 39/12
C12N 7/01
Applicants: FUNDAÇÃO OSWALDO CRUZ
Inventors: BONALDO, Myrna, Cristina
LIMA, Noemia, Santana
Title: HETEROLOGOUS EXPRESSION CASSETTE, DNA CONSTRUCT AND VACCINE COMPOSITION FOR IMMUNIZING AGAINST FLAVIVIRUS AND/OR OTHER PATHOGENS
Abstract:
The present invention relates to DNA constructs, recombinant viruses and vaccine compositions containing the recombinant viruses obtained. This invention also concerns an improvement made to the expression vector of the live attenuated virus of yellow fever 17D. The present invention provides for the introduction of modifications to the expression cassette of heterologous proteins in the intergenic region E/NS1 of the vaccine virus of yellow fever 17D. The two new functional domains inserted into the expression cassette were (1) a coding sequence for N-glycosylation motif, located between the N-terminal NS1 motif and the heterologous protein and (2) a sequence that promotes the proteolytic cleavage or otherwise of the recombinant protein in order to release same from the C-terminal of same containing the transmembrane domains and, consequently, from the association thereof with the membrane of the endoplasmic reticulum RE.