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1. (WO2018025207) BIOLOGICAL INDICATOR FOR DISINFECTION PROCESS CHALLENGE DEVICE

Pub. No.:    WO/2018/025207    International Application No.:    PCT/IB2017/054737
Publication Date: Fri Feb 09 00:59:59 CET 2018 International Filing Date: Thu Aug 03 01:59:59 CEST 2017
IPC: A61L 2/28
Applicants: 3M INNOVATIVE PROPERTIES COMPANY
Inventors: BENNAARS-EIDEN, Assumpta A. G.
BOMMARITO, G. Marco
BONILLA, Tonya D.
CONNELL, Jodi L.
DUDA, Ruthann R.
SGOLASTRA, Federica
VAIL, Andrew W.
Title: BIOLOGICAL INDICATOR FOR DISINFECTION PROCESS CHALLENGE DEVICE
Abstract:
A method of verifying the efficacy of a disinfection process is provided. The method includes flowing a liquid disinfectant into an indicator device that has a plurality of indicator microorganisms disposed therein, the indicator microorganisms comprising or capable of producing a detectable enzyme activity; contacting the indicator microorganisms with the liquid disinfectant for a predetermined minimum first period of time at a first temperature; after contacting the indicator microorganisms with the liquid disinfectant for the first period of time at the first temperature, contacting the indicator microorganisms for a second period of time with a detection medium comprising a detection reagent; and during or after the second period of time, detecting a quantity of the detectable enzyme activity that was not inactivated by the contact with the disinfectant. The indicator device is also provided.