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|1. (WO2018024153) PREPARATION METHOD AND USE OF RECOMBINANT SWINE FEVER E2 PROTEIN AND SUBUNIT VACCINE THEREOF|
|Applicants:||NOVO BIOTECH CORP.
|Title:||PREPARATION METHOD AND USE OF RECOMBINANT SWINE FEVER E2 PROTEIN AND SUBUNIT VACCINE THEREOF|
Disclosed are a preparation method and use of a recombinant swine fever E2 protein and subunit vaccine thereof. The method for preparing the recombinant swine fever E2 protein comprises the following steps: 1) cloning the swine fever E2 protein coding gene into an eukaryotic expression vector to obtain a recombinant plasmid containing the swine fever E2 protein coding gene; 2) transfecting the recombinant plasmid containing the swine fever E2 protein coding gene into a CHO cell strain; 3) obtaining a highly expressed cell strain by culturing, screening and domestication of the CHO cell strain described in step 2); and 4) fermenting and culturing the cell strain described in step 3), and obtaining the recombinant swine fever E2 protein after purification. The method can harvest the target protein from the cell culture supernatant with a yield of up to 1 g/L, and not only shortens the protein purification time and simplifies the vaccine production steps, but also reduces the vaccine production cost.