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1. (WO2018024153) PREPARATION METHOD AND USE OF RECOMBINANT SWINE FEVER E2 PROTEIN AND SUBUNIT VACCINE THEREOF

Pub. No.:    WO/2018/024153    International Application No.:    PCT/CN2017/094651
Publication Date: Fri Feb 09 00:59:59 CET 2018 International Filing Date: Fri Jul 28 01:59:59 CEST 2017
IPC: C12N 15/85
C12N 15/40
C07K 14/18
A61K 39/187
A61P 31/14
Applicants: NOVO BIOTECH CORP.
浙江海隆生物科技有限公司
Inventors: QIAN, Hong
钱泓
WU, Youqiang
吴有强
CHE, Ying
车影
WU, Sufang
吴素芳
ZHA, Yinhe
查银河
Title: PREPARATION METHOD AND USE OF RECOMBINANT SWINE FEVER E2 PROTEIN AND SUBUNIT VACCINE THEREOF
Abstract:
Disclosed are a preparation method and use of a recombinant swine fever E2 protein and subunit vaccine thereof. The method for preparing the recombinant swine fever E2 protein comprises the following steps: 1) cloning the swine fever E2 protein coding gene into an eukaryotic expression vector to obtain a recombinant plasmid containing the swine fever E2 protein coding gene; 2) transfecting the recombinant plasmid containing the swine fever E2 protein coding gene into a CHO cell strain; 3) obtaining a highly expressed cell strain by culturing, screening and domestication of the CHO cell strain described in step 2); and 4) fermenting and culturing the cell strain described in step 3), and obtaining the recombinant swine fever E2 protein after purification. The method can harvest the target protein from the cell culture supernatant with a yield of up to 1 g/L, and not only shortens the protein purification time and simplifies the vaccine production steps, but also reduces the vaccine production cost.