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1. (WO2018022628) AFFINITY CHROMATOGRAPHY WASH BUFFER
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/022628 International Application No.: PCT/US2017/043743
Publication Date: 01.02.2018 International Filing Date: 25.07.2017
IPC:
C07K 1/22 (2006.01) ,A61K 39/395 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
1
General processes for the preparation of peptides
14
Extraction; Separation; Purification
16
by chromatography
22
Affinity chromatography or related techniques based upon selective absorption processes
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
39
Medicinal preparations containing antigens or antibodies
395
Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
Applicants: CEPHALON, INC.[US/US]; 41 Moores Road Frazer, Pennsylvania 19355, US
Inventors: WANG, Lu; US
ZHOU, Tianyi; US
ZHANG, Zhaoqing; US
JIN, Mi; US
Agent: SHEA, JR, Timothy J.; US
NANNENGA-COMBS, Bonnie; US
BEZOS, Salvador M.; US
BODENSTEIN, Matthew; US
CALVO, Paul A.; US
CAMARCE, Christian; US
CIMBALA, Michelle A.; US
COLLER III, Richard D.; US
CONKLIN, Kyle; US
CORNWELL, David K.S.; US
COVERT, John M.; US
DURKIN, Tracy-Gene G.; US
EISENBERG, Jason D.; US
ELLISON, Eldora L.; US
ESMOND, Robert W.; US
FEATHERSTONE, Donald J.; US
FRUEAUF, Jeremiah B.; US
GOLDSTEIN, Jorge A.; US
GORDON, Lori A.; US
HELVEY, Jeffrey T.; US
HICKS, Ross; US
HOLOUBEK, Michelle K.; US
HOOVER, Kenley K.; US
JACKMAN, Peter A.; US
KIM, Ji-Eun; US
LEE, Michael Q.; US
LONGSWORTH, Gaby L.; US
MCGROARTY, John P.; US
MESSINGER, Michael V.; US
MILLONIG, Robert C.; US
MUTSCHELKNAUS, Joseph; US
PICKARD, Byron L.; US
RAY, Michael B.; US
ROSE GILLENTINE, Marsha; US
RYGIEL, Mark W.; US
SOKOHL, Robert E.; US
SPECHT, Michael D.; US
STEFFE, Eric K.; US
STERLING, Deborah A.; US
STERNE, Robert Greene; US
TUMINARO, Jonathan; US
WRIGHT, Jon E.; US
Priority Data:
62/366,30225.07.2016US
62/366,30925.07.2016US
62/523,03221.06.2017US
Title (EN) AFFINITY CHROMATOGRAPHY WASH BUFFER
(FR) TAMPON DE LAVAGE POUR CHROMATOGRAPHIE PAR AFFINITÉ
Abstract:
(EN) A wash buffer comprising greater than 0 mM and less than about 500 mM arginine, greater than 0 mM and less than about 250 mM guanidine, greater than 0 mM and less than about 250 mM sodium chloride, and greater than 0 mM and less than about 50 mM of an anionic surfactant, or greater than 0% and less than about 0.25% w/v of a non-ionic surfactant. When used during affinity chromatography purification of a protein of interest, such as an antibody, the wash buffer significantly reduces the level of host cell proteins from the preparation. Following affinity chromatography with the wash buffer, the protein of interest may be further purified using membrane chromatography.
(FR) La présente invention concerne un tampon de lavage contenant plus de 0 mM et moins d'environ 500 mM d'arginine, plus de 0 mM et moins d'environ 250 mM de guanidine, plus de 0 mM et moins d'environ 250 mM de chlorure de sodium, et plus de 0 mM et moins d'environ 50 mM d'un tensioactif anionique, ou plus de 0 % et moins d'environ 0,25 % en poids/volume d'un tensioactif non ionique. Quand il est utilisé pendant la purification par chromatographie par affinité d'une protéine d'intérêt, telle qu'un anticorps, le tampon de lavage réduit significativement le taux des protéines provenant d'une cellule hôte dans la préparation. Après la chromatographie par affinité avec le tampon de lavage, la protéine d'intérêt peut être davantage purifiée par chromatographie sur membrane.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)