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1. (WO2018021653) REPORTER CELL LINE IN WHICH IL-7 IS EXPRESSED AND METHOD FOR SCREENING FOR IMMUNODEFICIENCY DISEASE THERAPEUTIC AGENTS BY USING SAME
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Pub. No.: WO/2018/021653 International Application No.: PCT/KR2017/003213
Publication Date: 01.02.2018 International Filing Date: 24.03.2017
IPC:
C12N 15/85 (2006.01) ,C12N 15/65 (2006.01) ,C07K 14/54 (2006.01) ,G01N 33/50 (2006.01)
Applicants: UNIVERSITY OF ULSAN FOUNDATION FOR INDUSTRY COOPERATION[KR/KR]; 93 Daehak-ro Nam-gu Ulsan 44610, KR
THE ASAN FOUNDATION[KR/KR]; (Pungnap-dong), 88, Olympic-ro 43-gil Songpa-gu Seoul 05505, KR
Inventors: LEE, Myeong Sup; KR
CHO, Yeon Sook; KR
KIM, Byung Soo; KR
Agent: TAEBAEK INTELLECTUAL PROPERTY LAW FIRM; #601 Innoplex 1 cha, 151Gasandigital 1-ro Geumcheon-gu Seoul 08506, KR
Priority Data:
10-2016-009477026.07.2016KR
Title (EN) REPORTER CELL LINE IN WHICH IL-7 IS EXPRESSED AND METHOD FOR SCREENING FOR IMMUNODEFICIENCY DISEASE THERAPEUTIC AGENTS BY USING SAME
(FR) LIGNÉE CELLULAIRE RAPPORTEUR DANS LAQUELLE L'IL-7 EST EXPRIMÉE ET PROCÉDÉ DE CRIBLAGE D'AGENTS THÉRAPEUTIQUES POUR UNE MALADIE LIÉE À L'IMMUNODÉFICIENCE À L'AIDE DE CELLE-CI
(KO) IL-7 발현 리포터 세포주 및 이를 이용한 면역결핍 질환 치료제 스크리닝 방법
Abstract: front page image
(EN) The present invention relates to a reporter cell line in which IL-7 is expressed and a method for screening for immunodeficiency disease therapeutic agents by using the same. The present inventors have developed a reporter system for observing the internal expression of IL-7, at the transcriptional level, in a human cell line by using Cas9 gene-targeting technology. In addition, the present inventors have carried out a pilot screening, in an HTS format, for compounds inducing the up-regulation of IL-7, and have discovered a compound enabling the transcription of an IL-7 gene to be up-regulated. Therefore, the IL-7 reporter system and IL-7 reporter cell line of the present invention can be very useful in screening, in an HTS format, for compounds regulating IL-7.
(FR) La présente invention concerne une lignée cellulaire rapporteur dans laquelle l'IL-7 est exprimée et un procédé de criblage d'agents thérapeutiques pour une maladie liée à l'immunodéficience à l'aide de celle-ci. Les présents inventeurs ont développé un système rapporteur pour observer l'expression interne de l'IL-7, au niveau transcriptionnel, dans une lignée cellulaire humaine en utilisant la technologie de ciblage du gène Cas9. En outre, les présents inventeurs ont effectué un criblage pilote, dans un format HTS, à la recherche de composés induisant la régulation à la hausse de l'IL-7, et ont découvert un composé permettant de réguler à la hausse la transcription d'un gène de l'IL-7. Par conséquent, le système rapporteur de l'IL-7 et la lignée cellulaire rapporteur de l'IL-7 de la présente invention peuvent être très utiles dans le criblage, dans un format HTS, de composés régulant l'IL-7.
(KO) 본 발명은 IL-7 발현 리포터 세포주 및 이를 이용한 면역결핍 질환 치료제 스크리닝 방법에 관한 것으로서, 본 발명자들은 Cas9 유전자-표적화 기술을 이용하여, 인간 세포주에서 전사 수준의 내재적 IL-7 발현을 관측하기 위한 리포터 시스템을 개발하였다. 또한, 본 발명자들은 HTS 포맷에서 IL-7 상향 조절을 유도하는 화합물에 대한 파일럿-스크리닝을 수행하였고, IL-7 유전자 전사를 상향조절할 수 있는 화합물을 발굴하였다. 따라서, 본 발명의 IL-7 리포터 시스템 및 IL-7 리포터 세포주는 HTS 포맷에서 IL-7 조절 화합물을 스크리닝하는데 매우 유용하게 활용될 수 있다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Korean (KO)
Filing Language: Korean (KO)