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1. (WO2018021366) CELL PREPARATION METHOD, CELL CULTIVATION DEVICE, AND KIT
PCT Biblio. Data
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National Phase
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Pub. No.:
WO/2018/021366
International Application No.:
PCT/JP2017/026946
Publication Date:
01.02.2018
International Filing Date:
25.07.2017
IPC:
C12M 1/00
(2006.01),
B29C 41/12
(2006.01),
B32B 3/12
(2006.01),
B32B 5/32
(2006.01),
B32B 27/34
(2006.01),
C08J 9/28
(2006.01),
C12M 3/00
(2006.01),
C12M 3/04
(2006.01),
C12N 1/00
(2006.01),
C12N 5/00
(2006.01),
C12N 5/04
(2006.01),
C12N 5/07
(2010.01),
C12N 5/10
(2006.01)
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1
Apparatus for enzymology or microbiology
B
PERFORMING OPERATIONS; TRANSPORTING
29
WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
C
SHAPING OR JOINING OF PLASTICS; SHAPING OF SUBSTANCES IN A PLASTIC STATE, IN GENERAL; AFTER- TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
41
Shaping by coating a mould, core or other substrate, i.e. by depositing material and stripping-off the shaped article; Apparatus therefor
02
for making articles of definite length, i.e. discrete articles
12
Spreading-out the material on a substrate
B
PERFORMING OPERATIONS; TRANSPORTING
32
LAYERED PRODUCTS
B
LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
3
Layered products essentially comprising a layer with external or internal discontinuities or unevennesses, or a layer of non-planar form; Layered products essentially having particular features of form
10
characterised by a discontinuous layer, i.e. apertured or formed of separate pieces of material
12
characterised by a layer of regularly-arranged cells whether integral or formed individually or by conjunction of separate strips, e.g. honeycomb structure
B
PERFORMING OPERATIONS; TRANSPORTING
32
LAYERED PRODUCTS
B
LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
5
Layered products characterised by the non-homogeneity or physical structure of a layer
22
characterised by the presence of two or more layers which comprise fibres, filaments, granules, or powder, or are foamed or specifically porous
32
both layers being foamed or specifically porous
B
PERFORMING OPERATIONS; TRANSPORTING
32
LAYERED PRODUCTS
B
LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
27
Layered products essentially comprising synthetic resin
34
comprising polyamides
C
CHEMISTRY; METALLURGY
08
ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
J
WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H142
9
Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
28
by elimination of a liquid phase from a macromolecular composition or article, e.g. drying of coagulum
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3
Tissue, human, animal or plant cell, or virus culture apparatus
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
M
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3
Tissue, human, animal or plant cell, or virus culture apparatus
04
with means providing thin layers
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
04
Plant cells or tissues
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
Applicants:
UBE INDUSTRIES, LTD.
[JP/JP]; 1978-96, Oaza Kogushi, Ube-shi, Yamaguchi 7558633 (JP)
Inventors:
HAGIHARA, Masahiko
; (JP).
OHYA, Shyusei
; (JP)
Agent:
AOKI, Atsushi
; (JP).
WATANABE, Yoichi
; (JP).
DENO, Satoru
; (JP).
MITSUHASHI, Shinji
; (JP)
Priority Data:
2016-145822
25.07.2016
JP
Title
(EN)
CELL PREPARATION METHOD, CELL CULTIVATION DEVICE, AND KIT
(FR)
PROCÉDÉ DE PRÉPARATION DE CELLULES, DISPOSITIF DE CULTURE CELLULAIRE ET KIT
(JA)
細胞の調製方法、細胞培養装置及びキット
Abstract:
(EN)
The present invention relates to a cell preparation method that includes a step in which cells are applied to a polyimide porous film and cultivated, wherein the polyimide porous film is a polyimide porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, and the polyimide porous film is produced by a method including the following steps: (1) a step in which a polyamic acid solution comprising polyamic acid and an organic polar solvent is flow cast in a film shape and the result is immersed in or brought into contact with a coagulation medium to create a porous film of polyamic acid; and (2) a step in which the porous film of polyamic acid obtained in step (1) is heat-treated and imidized.
(FR)
La présente invention concerne un procédé de préparation de cellules qui comprend une étape dans laquelle des cellules sont appliquées sur un film en polyimide poreux et cultivées, le film en polyimide poreux étant un film en polyimide poreux ayant une structure à trois couches, ayant une couche de surface A et une couche de surface B qui ont une pluralité de trous, et une couche de macrovides qui est prise en sandwich entre la couche de surface A et la couche de surface B, et le film en polyimide poreux est produit par un procédé comprenant les étapes suivantes : (1) une étape dans laquelle une solution d'acide polyamique comprenant de l'acide polyamique et un solvant polaire organique est moulée en une forme de film et le résultat est immergé ou mis en contact avec un milieu de coagulation pour créer un film poreux d'acide polyamique ; et (2) une étape dans laquelle le film poreux d'acide polyamique obtenu à l'étape (1) est soumis à un traitement thermique et imidisé.
(JA)
本発明は、細胞の調製方法であって、細胞をポリイミド多孔質膜に適用し、培養する工程を含み、ここで前記ポリイミド多孔質膜は、複数の孔を有する表面層A及び表面層Bと、前記表面層A及び表面層Bの間に挟まれたマクロボイド層とを有する三層構造のポリイミド多孔質膜であり、以下の工程:(1)ポリアミック酸と有機極性溶媒とからなるポリアミック酸溶液をフィルム状に流延し、凝固溶媒に浸漬又は接触させて、ポリアミック酸の多孔質膜を作製する工程、及び(2)前記工程(1)で得られたポリアミック酸の多孔質膜を熱処理してイミド化する工程を含む方法で製造されるポリイミド多孔質膜である、細胞の調製方法に関する。
Designated States:
AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language:
Japanese (
JA
)
Filing Language:
Japanese (
JA
)