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1. (WO2018021357) METHOD TO SUPPRESS STEM CELL DIFFERENTIATION, METHOD TO PREPARE STEM CELLS, AND METHOD TO INDUCE DIFFERENTIATION OF STEM CELLS
PCT Biblio. Data
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Pub. No.:
WO/2018/021357
International Application No.:
PCT/JP2017/026937
Publication Date:
01.02.2018
International Filing Date:
25.07.2017
IPC:
C12N 5/00
(2006.01),
C12N 5/07
(2010.01),
C12N 5/0735
(2010.01),
C12N 5/074
(2010.01),
C12N 5/0775
(2010.01),
C12N 5/0789
(2010.01),
C12N 5/10
(2006.01)
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
073
Embryonic cells or tissues; Foetal cells or tissues
0735
Embryonic stem cells; Embryonic germ cells
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
074
Adult stem cells
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
077
Mesenchymal cells, e.g. bone cells, cartilage cells, marrow stromal cells, fat cells or muscle cells
0775
Mesenchymal stem cells; Adipose-tissue derived stem cells
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
078
Cells from blood or from the immune system
0789
Stem cells; Multipotent progenitor cells
C
CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10
Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
Applicants:
UBE INDUSTRIES, LTD.
[JP/JP]; 1978-96, Oaza Kogushi, Ube-shi, Yamaguchi 7558633 (JP)
Inventors:
HAGIHARA, Masahiko
; (JP).
KAWAGUCHI, Tetsuo
; (JP).
BABA, Kousuke
; (JP)
Agent:
AOKI, Atsushi
; (JP).
WATANABE, Yoichi
; (JP).
DENO, Satoru
; (JP).
MITSUHASHI, Shinji
; (JP)
Priority Data:
2016-145831
25.07.2016
JP
Title
(EN)
METHOD TO SUPPRESS STEM CELL DIFFERENTIATION, METHOD TO PREPARE STEM CELLS, AND METHOD TO INDUCE DIFFERENTIATION OF STEM CELLS
(FR)
PROCÉDÉ DE SUPPRESSION DE LA DIFFÉRENCIATION DE CELLULES SOUCHES, PROCÉDÉ DE PRÉPARATION DE CELLULES SOUCHES, ET PROCÉDÉ D’INDUCTION DE LA DIFFÉRENCIATION DE CELLULES SOUCHES
(JA)
幹細胞の分化を抑制する方法、幹細胞を調製する方法、及び幹細胞を分化誘導する方法
Abstract:
(EN)
The present invention relates to a method to suppress stem cell differentiation, said method including: (1) a step in which the stem cells are applied to a polymer porous film; and (2) a step in which the stem cells are cultivated and allowed to multiply, wherein the polymer porous film is a polymer porous film with a three-layer structure, having a surface layer A and a surface layer B that have a plurality of holes, and a macrovoid layer that is sandwiched between the surface layer A and the surface layer B, the average hole diameter of the holes present in the surface layer A is smaller than the average hole diameter of the holes present in the surface layer B, the macrovoid layer has dividing walls that are connected to the surface layers A and B, and a plurality of macrovoids that are surrounded by the dividing walls and the surface layers A and B, and the holes in the surface layers A and B are in communication with the macrovoids.
(FR)
La présente invention concerne un procédé de suppression de la différenciation de cellules souches, ledit procédé comprenant : (1) une étape dans laquelle les cellules souches sont appliquées à un film polymère poreux ; et (2) une étape dans laquelle les cellules souches sont cultivées et amenées à se multiplier, le film polymère poreux étant un film polymère poreux avec une structure à trois couches, possédant une couche de surface (A) et une couche de surface (B) qui possèdent une pluralité de trous, et une couche de macrovides qui est prise en sandwich entre la couche de surface (A) et la couche de surface (B), le diamètre moyen de trou des trous présents dans la couche de surface (A) est inférieur au diamètre moyen de trou des trous présents dans la couche de surface (B), la couche de macrovides possède des parois de séparation qui sont reliées aux couches de surface (A) et (B), et une pluralité de macrovides qui sont entourés des parois de séparation et des couches de surface (A) et (B), et les trous dans les couches de surface (A) et (B) se trouvent en communication avec les macrovides.
(JA)
本発明は、幹細胞の分化を抑制する方法であって、(1)前記幹細胞をポリマー多孔質膜に適用する工程、及び(2)前記幹細胞を培養し、増殖させる工程を含み、ここで、前記ポリマー多孔質膜が、複数の孔を有する表面層A及び表面層Bと、前記表面層A及び表面層Bの間に挟まれたマクロボイド層とを有する三層構造のポリマー多孔質膜であり、ここで前記表面層Aに存在する孔の平均孔径は、前記表面層Bに存在する孔の平均孔径よりも小さく、前記マクロボイド層は、前記表面層A及びBに結合した隔壁と、当該隔壁並びに前記表面層A及びBに囲まれた複数のマクロボイドとを有し、前記表面層A及びBにおける孔が前記マクロボイドに連通している、前記方法に関する。
Designated States:
AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language:
Japanese (
JA
)
Filing Language:
Japanese (
JA
)