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1. (WO2018018613) CELL CULTURE MEDIUM AND CULTURE METHOD FOR INCREASING PURITY OF ANTIBODY
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.: WO/2018/018613 International Application No.: PCT/CN2016/092322
Publication Date: 01.02.2018 International Filing Date: 29.07.2016
IPC:
C12N 5/07 (2010.01) ,C12P 21/00 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
Applicants: SUNSHINE LAKE PHARMA CO., LTD.[CN/CN]; Northern Industrial Area, Songshan Lake Dongguan, Guangdong 523000, CN
Inventors: CHENG, Xiwen; CN
YAN, Chengwei; CN
YANG, Bin; CN
SUEN, Wen-Chen; CN
WENG, Yuancan; CN
DENG, Chongfei; CN
Priority Data:
Title (EN) CELL CULTURE MEDIUM AND CULTURE METHOD FOR INCREASING PURITY OF ANTIBODY
(FR) MILIEU DE CULTURE ET PROCÉDÉ DE CULTURE CELLULAIRES POUR AUGMENTER LA PURETÉ D'UN ANTICORPS
(ZH) 一种提高抗体纯度的细胞培养基和培养方法
Abstract:
(EN) Provided is a homemade supplemented medium that contains 90 mM to 500 mM of cysteine. Also provided is a cell culture method, comprising the steps of: inoculating a mammalian cell comprising a nucleic acid encoding an antibody into a basal medium; adding the homemade supplemented medium after entering the cellular proliferative stage; and monitoring the cell density to adjust the culture time.
(FR) L'invention concerne un milieu maison complété qui contient 90 mM à 500 mM de cystéine. L'invention porte également sur un procédé de culture cellulaire, comprenant les étapes consistant à : inoculer une cellule mammifère comprenant un acide nucléique codant pour un anticorps dans un milieu basal ; ajouter le milieu maison complété après l'entrée dans l'étage de prolifération cellulaire ; et surveiller la densité cellulaire pour ajuster le temps de culture.
(ZH) 提供了一种自制补料培养基,所述培养基含有90mM~500mM半胱氨酸。并提供了一种细胞培养方法,包括以下步骤:在基础培养基中接种包含编码抗体核酸的哺乳动物细胞;进入细胞增殖期后补加所述自制补料培养基;监测细胞密度调整培养时间。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)