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1. (WO2018016885) RNA DETECTING KIT AND METHOD

Pub. No.:    WO/2018/016885    International Application No.:    PCT/KR2017/007804
Publication Date: Fri Jan 26 00:59:59 CET 2018 International Filing Date: Fri Jul 21 01:59:59 CEST 2017
IPC: C12Q 1/68
Applicants: HEIMBIOTEK INC.
주식회사 하임바이오텍
Inventors: LEE, Jae Hoon
이재훈
Title: RNA DETECTING KIT AND METHOD
Abstract:
The present invention relates to a kit and a method for detecting an RNA molecule, particularly, a small-size RNA molecule such as miRNA. The present invention provides an RNA detecting kit and method in which short primers for reverse transcription are used in a reverse transcription process with the aim of rapidly diffusing the primers in a reverse-transcription reaction solution and thus reducing the reverse-transcription reaction time, enabling the overall PCR reaction rate to be increased and RNA to be detected stably and rapidly. In addition, the present invention provides an RNA detecting kit and method in which, after a reverse transcription process, extension is performed on a single-stranded cDNA by use of a primer for extension, far longer than a primer for reverse transcription, and thus a template long enough to conduct PCR amplification can be obtained.