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1. (WO2018016440) INTRODUCTION AGENT, KIT, SUBSTANCE INTRODUCTION METHOD, AND SCREENING METHOD

Pub. No.:    WO/2018/016440    International Application No.:    PCT/JP2017/025749
Publication Date: Fri Jan 26 00:59:59 CET 2018 International Filing Date: Sat Jul 15 01:59:59 CEST 2017
IPC: C12N 1/00
C12N 15/09
Applicants: THE UNIVERSITY OF TOKYO
国立大学法人東京大学
Inventors: MURATA Masayuki
村田 昌之
KANO Fumi
加納 ふみ
MURAKAMI Masataka
村上 真隆
Title: INTRODUCTION AGENT, KIT, SUBSTANCE INTRODUCTION METHOD, AND SCREENING METHOD
Abstract:
This introduction agent includes a cholesterol-dependent cytolysin, for which the optimum membrane perforation activity pH at 30-40°C is at least 0 but less than 6, and is used in the introduction of a substance into a cell. This kit comprises the introduction agent and is used in the introduction of a substance into a cell. This substance introduction method includes a step 1 for perforating a cell membrane by bringing the introduction agent into contact with the cell and for introducing a substance into the cell. This screening method for a substance uses the substance introduction method, and the substance introduced into the cell is the substance to be screened.