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1. (WO2018013990) SCARLESS DNA ASSEMBLY AND GENOME EDITING USING CRISPR/CPF1 AND DNA LIGASE

Pub. No.:    WO/2018/013990    International Application No.:    PCT/US2017/042245
Publication Date: Fri Jan 19 00:59:59 CET 2018 International Filing Date: Sat Jul 15 01:59:59 CEST 2017
IPC: C12N 15/09
C12N 15/11
C12N 15/113
C12N 15/63
C12N 15/66
Applicants: ZYMERGEN INC.
Inventors: DELOACHE, William C.
MARINUS VAN ROSSUM, Hendrik
PATEL, Kedar Gautam
Title: SCARLESS DNA ASSEMBLY AND GENOME EDITING USING CRISPR/CPF1 AND DNA LIGASE
Abstract:
The disclosure describes a scarless DNA assembly and genome editing methodology termed "CLIC" (CRISPR and Ligase Cloning), which utilizes a CRISPR/Cpfl complex and DNA ligase to perform programmable gene editing and nucleotide assembly. The CLIC process is highly amenable to applications in vitro for the scarless assembly of a plurality of DNA parts simultaneously or in vivo for the site-specific insertion of one or more DNA molecules into the host genome.