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1. (WO2018012827) CULTURING METHOD FOR CULTURED LEGUMINOUS ROOTS HAVING INCREASED COUMESTROL CONTENT

Pub. No.:    WO/2018/012827    International Application No.:    PCT/KR2017/007356
Publication Date: Fri Jan 19 00:59:59 CET 2018 International Filing Date: Tue Jul 11 01:59:59 CEST 2017
IPC: A01H 4/00
A01H 5/10
C12N 5/00
C12N 5/04
Applicants: AMOREPACIFIC CORPORATION
(주)아모레퍼시픽
CHUNGBUK NATIONAL UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
충북대학교 산학협력단
Inventors: LEE, Eun Jung
이은정
KANG, Young-Gyu
강영규
PARK, Jun Seong
박준성
PARK, So Young
박소영
KIM, Young Eun
김영은
Title: CULTURING METHOD FOR CULTURED LEGUMINOUS ROOTS HAVING INCREASED COUMESTROL CONTENT
Abstract:
The present specification relates to a culturing method for cultured leguminous roots having an increased coumestrol content, the method being capable of mass-producing coumestrol, which is present in a very small amount in a leguminous plant, wherein the culturing method comprises the steps of: (a) germinating leguminous seeds in a culture medium to induce in vitro plants having cotyledons, hypocotyls, and radicles; (b) culturing, in a culture medium, at least one site of the cotyledons, hypocotyls, and radicles of the induced in vitro plant to induce site-specific cultured roots; and (c) multiplying the induced site-specific cultured roots in a culture medium, wherein the culture media contain nutrient components of NH4NO3, CaCl2·2H2O, MgSO4·7H2O, KH2PO4, and KNO3.