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1. (WO2018011166) METHODS FOR QUANTIFYING THE POPULATION OF MYELOID DENDRITIC CELLS IN A TISSUE SAMPLE

Pub. No.:    WO/2018/011166    International Application No.:    PCT/EP2017/067327
Publication Date: Fri Jan 19 00:59:59 CET 2018 International Filing Date: Wed Jul 12 01:59:59 CEST 2017
IPC: C12Q 1/68
Applicants: INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE)
UNIVERSITÉ PIERRE ET MARIE CURIE (PARIS 6)
UNIVERSITÉ PARIS DIDEROT - PARIS 7
UNIVERSITÉ PARIS DESCARTES
LIGUE NATIONALE CONTRE LE CANCER
Inventors: FRIDMAN, Wolf Herman
BECHT, Etienne
DE REYNIES, Aurélien
Title: METHODS FOR QUANTIFYING THE POPULATION OF MYELOID DENDRITIC CELLS IN A TISSUE SAMPLE
Abstract:
The present invention relates to methods for quantifying the population of myeloid dendritic cells in a tissue sample. The tumor microenvironment is a complex tissue which contains many distinct cell populations, and whose composition may predict prognosis and response to therapies. In the present disclosure, using samples of purified cell populations, the inventors defined specific and robust transcriptomic markers of the immune and stromal cell populations of the tumor microenvironment, and they quantitatively validated them in an in-vitro RNA mixture model. More, they developed the 'Microenvironment Cell Populations-counter' (MCP-counter) method, which, using transcriptomic data, allows a robust quantification of the abundance of immune and stromal populations in cellularly heterogeneous healthy and cancerous tissues. Thus, the present invention relates to a method for quantifying the population of myeloid dendritic cells in a tissue sample obtained from a subject comprising determining the expression level of at least one gene selected from the group consisting of CD1E, CLEC10A, CLIC2, CD1A, WFDC21P and CD1B.