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1. (WO2018009809) EXTRACHROMOSOMAL SWITCHING AUXOTROPHIES PROGRESSIVELY BY INTEGRATION (ESWAP-IN) FOR ASSEMBLY OF DNA SEQUENCES IN YEAST
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.:    WO/2018/009809    International Application No.:    PCT/US2017/041117
Publication Date: 11.01.2018 International Filing Date: 07.07.2017
IPC:
C12N 1/16 (2006.01), C12N 1/19 (2006.01), C12N 5/10 (2006.01), C12N 15/85 (2006.01), C12N 15/90 (2006.01), C12N 15/81 (2006.01), C12N 15/63 (2006.01), C12N 15/64 (2006.01), C12N 15/65 (2006.01), C12N 15/66 (2006.01), C12P 19/34 (2006.01)
Applicants: NEW YORK UNIVERSITY [US/US]; 70 Washington Square S. New York, NY 10012 (US)
Inventors: BOEKE, Jef, D.; (US).
MITCHELL, Leslie, A.; (US).
AGMON, Neta; (US)
Agent: WATT, Rachel, S.; (US).
ROMAN, Paul, J.; (US).
CUTAIA, Alfonzo, I.; (US).
KADLE, Ranjana; (US).
LOPINSKI, John, D.; (US)
Priority Data:
62/359,264 07.07.2016 US
Title (EN) EXTRACHROMOSOMAL SWITCHING AUXOTROPHIES PROGRESSIVELY BY INTEGRATION (ESWAP-IN) FOR ASSEMBLY OF DNA SEQUENCES IN YEAST
(FR) AUXOTROPHIES À COMMUTATION EXTRACHROMOSOMIQUE PAR INTÉGRATION (ESWAP-IN) POUR L’ASSEMBLAGE DE SÉQUENCES D’ADN DANS LA LEVURE
Abstract: front page image
(EN)Compositions, methods and kits are provided. The compositions, methods and kits are for assembly of series of DNA segments in yeast using homologous recombination. The assembled DNA segments are maintained episomally. Yeast made using the methods are included, as are methods of using the yeast to express proteins, and for screening test agents that can affect yeast that are modified to include the assembled DNA segments.
(FR)L’invention concerne des compositions, des procédés et des trousses. Les compositions, procédés et trousses servent à l’assemblage de séries de segments d’ADN dans la levure par recombinaison homologue. Les segments d’ADN assemblés sont maintenus de manière épisomique. La levure obtenue par les procédés est incluse, ainsi que des procédés d’utilisation de la levure pour exprimer des protéines, et pour l’analyse d’agents d’épreuve qui peuvent affecter la levure qui est modifiée pour inclure les segments d’ADN assemblés.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)