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1. (WO2018008903) ADIPOCYTE-TARGETING NON-VIRAL GENE DELIVERY COMPLEX COMPRISING DUAL PLASMID VECTOR
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Pub. No.: WO/2018/008903 International Application No.: PCT/KR2017/006963
Publication Date: 11.01.2018 International Filing Date: 30.06.2017
IPC:
C12N 15/85 (2006.01) ,C12N 15/113 (2010.01) ,A61K 48/00 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
85
for animal cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
113
Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
A HUMAN NECESSITIES
61
MEDICAL OR VETERINARY SCIENCE; HYGIENE
K
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
48
Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
Applicants:
한양대학교 산학협력단 INDUSTRY-UNIVERSITY COOPERATION FOUNDATION HANYANG UNIVERSITY [KR/KR]; 서울시 성동구 왕십리로 222 222 Wangsimni-ro Seongdong-gu Seoul 04763, KR
Inventors:
김용희 KIM, Yong-Hee; KR
김형진 KIM, Hyung Jin; KR
Agent:
특허법인 아이시스 ISIS IP LAW LLC; 서울시 강남구 테헤란로 63길 14, 8층 8F., 14, Teheran-ro 63-gil Gangnam-gu Seoul 06160, KR
Priority Data:
10-2016-008463105.07.2016KR
Title (EN) ADIPOCYTE-TARGETING NON-VIRAL GENE DELIVERY COMPLEX COMPRISING DUAL PLASMID VECTOR
(FR) COMPLEXE D'ADMINISTRATION DE GÈNE NON VIRAL CIBLANT LES ADIPOCYTES COMPRENANT UN VECTEUR PLASMIDIQUE DOUBLE
(KO) 이중 플라스미드 벡터를 포함하는 지방세포 표적 비바이러스성 유전자 전달복합체
Abstract:
(EN) The present invention relates to: an adipocyte-targeting non-viral gene delivery complex comprising a sh(FABP4 + FABP5) dual plasmid vector; and treatment for obesity and obesity-induced metabolic syndromes by using the same and, more particularly, to a gene delivery complex comprising: an adipocyte-targeting sequence; an arginine 9 (R9) peptide; and a dual plasmid vector comprising a gene for treatment of obesity and obesity-induced metabolic syndromes, wherein the gene for treatment of obesity and obesity-induced metabolic syndromes is a base sequence inhibiting the expression of a FABP4 gene and a FABP5 gene. According to the present invention, in order to treat obesity-related diseases, a dual plasmid vector capable of simultaneously inhibiting the FABP4 and FABP5 genes is produced, and since a gene delivery complex is provided by binding the vector to a predetermined delivery system specifically delivering the same to adipocytes, an excellent and non-toxic obesity treatment effect targeting only adipocytes can be achieved.
(FR) La présente invention concerne : un complexe d'administration de gène non viral ciblant les adipocytes comprenant un vecteur plasmidique double sh(FABP4 + FABP5) ; et un traitement de l'obésité et des syndromes métaboliques induits par l'obésité à l'aide de celui-ci et, plus particulièrement, un complexe d'administration de gène comprenant : une séquence ciblant les adipocytes ; un peptide d'arginine 9 (R9) ; et un vecteur plasmidique double comprenant un gène pour le traitement de l'obésité et des syndromes métaboliques induits par l'obésité, le gène pour le traitement de l'obésité et des syndromes métaboliques induits par l'obésité étant une séquence de bases inhibant l'expression d'un gène FABP4 et d'un gène FABP5. Selon la présente invention, afin de traiter des maladies liées à l'obésité, un vecteur plasmidique double capable d'inhiber simultanément les gènes FABP4 et FABP5 est produit et, étant donné qu'un complexe d'administration de gène est obtenu par la liaison du vecteur à un système d'administration prédéterminé qui l'administre en particulier aux adipocytes, on peut obtenir un excellent effet de traitement de l'obésité, non toxique, ciblant uniquement les adipocytes.
(KO) 본 발명은 sh(FABP4 + FABP5) 이중 플라스미드 벡터를 포함하는 지방세포 표적 비바이러스성 유전자 전달 복합체 및 이를 이용한 비만 및 비만 유래 대사증후군 치료에 관한 것으로서, 더욱 구체적으로는, 지방세포 표적 서열; R9 (아르기닌) 펩타이드; 및 비만 및 비만 유래 대사증후군 치료 유전자를 포함하는 이중 플라스미드 벡터를 포함하는 유전자 전달 복합체로서, 상기 비만 및 비만 유래 대사증후군 치료 유전자는 FABP4 유전자 및 FABP5의 유전자의 발현을 억제하는 염기 서열인 것을 특징으로 하는 유전자 전달 복합체에 관한 것이다. 본 발명에 따르면, 비만 관련 질환의 치료를 위해서 FABP4 유전자 및 FABP5 유전자를 동시에 억제할 수 있는 이중 플라스미드 벡터를 제작하였으며, 이를 지방세포에 특이적으로 전달하는 소정 전달체에 결합시켜 유전자 전달 복합체를 제공함으로써, 지방세포만을 대상으로 세포 독성 없이 우수한 비만 치료 효과를 달성할 수 있다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: Korean (KO)
Filing Language: Korean (KO)