Search International and National Patent Collections

1. (WO2018007575) GENOMIC SEQUENCES ENCODING FOR AN ATTENUATED MUTANT ZIKA VIRUS

Pub. No.:    WO/2018/007575    International Application No.:    PCT/EP2017/067059
Publication Date: Fri Jan 12 00:59:59 CET 2018 International Filing Date: Sat Jul 08 01:59:59 CEST 2017
IPC: C07K 14/005
C12N 7/04
C12N 15/113
Applicants: INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE)
UNIVERSITE DE LA REUNION SAINT DENIS
INSTITUT DE RECHERCHE POUR LE DÉVELOPPEMENT (IRD)
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
Inventors: GADEA, Gilles
GIRARDOT, Michael
DESPRES, Philippe
Title: GENOMIC SEQUENCES ENCODING FOR AN ATTENUATED MUTANT ZIKA VIRUS
Abstract:
The present invention relates to a genomic sequences encoding for an attenuated mutant Zika virus. The inventors have introduced some specific substitutions at very specific positions in the epidemic genomic sequence for restoring some fixation sites for miR-4279 that were originally present in the endemic genomic sequence. Moreover the inventors have additionally introduced mutation leading to the abrogation of the N-glycosylation site on the E protein which will prevent the generation of auto-antibodies responsible for Guillain-Barre syndrome. The inventors have produced additional mutations of the virus that result to a dramatic reduction of the cytopathic effects without affecting the capacity to produce high titers of virus. In particular the present invention relates to a genomic sequence characterized by the sequence represented by SEQ ID NO:1 wherein at least one site of fixation for miR-4279 is restored.