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1. (WO2018007469) METHOD FOR EXAMINING A SAMPLE, AND DEVICE FOR CARRYING OUT SUCH A METHOD

Pub. No.:    WO/2018/007469    International Application No.:    PCT/EP2017/066833
Publication Date: Fri Jan 12 00:59:59 CET 2018 International Filing Date: Thu Jul 06 01:59:59 CEST 2017
IPC: G02B 21/00
G02B 21/36
G02B 27/58
Applicants: LEICA MICROSYSTEMS CMS GMBH
Inventors: KNEBEL, Werner
FAHRBACH, Florian
Title: METHOD FOR EXAMINING A SAMPLE, AND DEVICE FOR CARRYING OUT SUCH A METHOD
Abstract:
The invention relates to a method for examining a sample, and to a device for carrying out such a method. The invention further relates to a device which is designed for examining a sample and which comprises at least one light source for producing an illumination light beam (1), having at least one wavelength suitable for the fluorescence excitation of the sample, and for producing a depletion or switching light beam (6), having at least one wavelength suitable for the depletion of the sample, and which further comprises at least one lens for focusing the illumination light beam (1) and the depletion or switching light beam (6). The device is characterized in that the illumination light beam (1) and the depletion or switching light beam (6) are guided in such a way that a sample to be examined is illuminated in a sample plane along a sample line with the illumination light beam (1) that propagates along the sample line and that the depletion or switching light beam (6) spatially overlaps the illumination light beam (1) in the sample plane at least to some extent; and in that an optical detection system is present and includes a detection lens and images the detection light emitted by the sample region along the sample line by means of the illumination light beam (1) onto a detection plane in which a detector is mounted that detects that portion of fluorescent light emitted by the sample plane as detected light that originates from a first subregion (19) of the overlapping region, in which the probability of interaction of the sample molecules with the depletion or switching light beam (6) is greater than 90%, in particular greater than 95%, very particularly preferably greater than 99%, and/or originates from a second subregion (20) that is surrounded by the first subregion (19) at least in sections and/or in which the depletion or switching light beam (6) has a zero point, while at the same time the detector blocks out and therefore does not detect at least to some extent the fluorescent light originating from outside the first and second subregion (19, 20).