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1. (WO2018007415) DEVICE AND METHOD FOR THE DETERMINATION OF TRANSFECTION

Pub. No.:    WO/2018/007415    International Application No.:    PCT/EP2017/066698
Publication Date: Fri Jan 12 00:59:59 CET 2018 International Filing Date: Wed Jul 05 01:59:59 CEST 2017
IPC: G01N 21/65
G01N 33/483
G01N 21/64
A61K 48/00
C12N 15/87
Applicants: CELLTOOL GMBH
Inventors: SCHÜTZE, Karin
SCHÜTZE, Raimund
Title: DEVICE AND METHOD FOR THE DETERMINATION OF TRANSFECTION
Abstract:
The present invention relates to an in vitro method for determining the transfection of a cell or group of cells, wherein said determination is performed spectroscopically. The determination comprises recording at least one Raman spectrum by means of Raman spectroscopy of the cell or group of cells. Furthermore, the present invention relates to a device for determining the transfection of a cell or group of cells, wherein the device comprises as a first unit (i) a microscope system in order to visualize the cells, as a second unit (ii) a Raman spectroscopy system in order to record a Raman spectrum of a cell or group of cells, and as a third unit (iii) an evaluation module which is coupled to the Raman spectroscopy system and which is configured to determine by means of the recorded Raman spectrum whether a cell or group of cells has been transfected. A statistical analysis of the Raman spectral data is disclosed using principal component analysis. In a PCA scores plot, it can be seen that the transfected samples differ from the control samples on the PC-1 axis.