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1. (WO2018005793) METHODS FOR GENERATING A GLUCOSE PERMEASE LIBRARY AND USES THEREOF

Pub. No.:    WO/2018/005793    International Application No.:    PCT/US2017/039997
Publication Date: Fri Jan 05 00:59:59 CET 2018 International Filing Date: Fri Jun 30 01:59:59 CEST 2017
IPC: C12N 15/70
C12P 19/02
Applicants: ZYMERGEN INC.
Inventors: MANCHESTER, Shawn
MELLIN, Jeffrey
Title: METHODS FOR GENERATING A GLUCOSE PERMEASE LIBRARY AND USES THEREOF
Abstract:
The present disclosure describes methods for generating microbial strains expressing a heterologous bacterial glucose permease gene that produce biomolecules of interest. In aspects, the disclosure provides novel bacterial strains, which express a heterologous bacterial glucose permease gene whose expression is controlled by a native Corynebacterium glutamicum promoter or a mutant promoter derived therefrom. Also provided herein are methods for producing a library of bacterial glucose permease genes using a promoter ladder comprising a plurality of promoters derived from Corynebacterium glutamicum.