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1. WO2018005720 - METHOD OF DETERMINING THE MOLECULAR BINDING BETWEEN LIBRARIES OF MOLECULES

Publication Number WO/2018/005720
Publication Date 04.01.2018
International Application No. PCT/US2017/039862
International Filing Date 29.06.2017
IPC
C12Q 1/68 2018.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
C07K 16/00 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins, e.g. monoclonal or polyclonal antibodies
C40B 30/04 2006.01
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
30Methods of screening libraries
04by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
C40B 40/08 2006.01
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
40Libraries per se, e.g. arrays, mixtures
04Libraries containing only organic compounds
06Libraries containing nucleotides or polynucleotides, or derivatives thereof
08Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
C40B 50/06 2006.01
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
50Methods of creating libraries, e.g. combinatorial synthesis
06Biochemical methods, e.g. using enzymes or whole viable microorganisms
CPC
C12N 15/1062
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
1034Isolating an individual clone by screening libraries
1062mRNA-Display, e.g. polypeptide and encoding template are connected covalently
C12Q 1/68
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
C12Q 1/6869
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6869Methods for sequencing
G01N 2458/10
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2458Labels used in chemical analysis of biological material
10Oligonucleotides as tagging agents for labelling antibodies
G01N 33/6845
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6845Methods of identifying protein-protein interactions in protein mixtures
Applicants
  • PRESIDENT AND FELLOWS OF HARVARD COLLEGE [US]/[US]
Inventors
  • GRISWOLD, Kettner John Frederick
  • FANG, Neng
Agents
  • IWANICKI, John P.
Priority Data
62/356,81130.06.2016US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD OF DETERMINING THE MOLECULAR BINDING BETWEEN LIBRARIES OF MOLECULES
(FR) PROCÉDÉ DE DÉTERMINATION DE LA LIAISON MOLÉCULAIRE ENTRE DES BANQUES DE MOLÉCULES
Abstract
(EN)
A method for determining the molecular binding between a target molecule library and a binder molecule library is provided including contacting the target molecule library comprising a plurality of mRNA-protein conjugates with the binder molecule library comprising a plurality of mRNA-protein conjugates under conditions where one or more proteins in the target library bind to one or more proteins in the binder library, and determining the molecular binding of the target and binder protein pairs by determining the sequences of the mRNAs conjugated to the target and binder protein pairs.
(FR)
L'invention porte sur un procédé visant à déterminer la liaison moléculaire entre une banque cible de molécules et une banque de molécules de liaison, et consistant à mettre en contact la banque cible de molécules comprenant une pluralité de conjugués ARNm-protéines avec la banque de molécules de liaison comprenant une pluralité de conjugués ARNm-protéines dans des conditions où une ou plusieurs protéines de la banque cible se lient à une ou plusieurs protéines de la banque de liants, et déterminer la liaison moléculaire des paires de protéines cibles et de liaison en déterminant les séquences des ARNm conjugués aux paires de protéines cibles et de liaison.
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