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1. (WO2018005655) METHODS FOR GENERATING A BACTERIAL HEMOGLOBIN LIBRARY AND USES THEREOF
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Pub. No.: WO/2018/005655 International Application No.: PCT/US2017/039772
Publication Date: 04.01.2018 International Filing Date: 28.06.2017
IPC:
C12N 15/09 (2006.01) ,C12N 15/67 (2006.01) ,C12N 15/52 (2006.01) ,C12N 15/70 (2006.01) ,C12N 15/10 (2006.01) ,C12N 15/77 (2006.01) ,C12N 15/82 (2006.01) ,C12P 21/02 (2006.01) ,C12P 19/34 (2006.01)
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
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BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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Recombinant DNA-technology
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Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
67
General methods for enhancing the expression
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Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
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DNA or RNA fragments; Modified forms thereof
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Genes encoding for enzymes or proenzymes
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Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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Recombinant DNA-technology
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Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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Vectors or expression systems specially adapted for E. coli
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Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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Recombinant DNA-technology
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Processes for the isolation, preparation or purification of DNA or RNA
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Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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Recombinant DNA-technology
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Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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for Corynebacterium; for Brevibacterium
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Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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Recombinant DNA-technology
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Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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Vectors or expression systems specially adapted for eukaryotic hosts
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for plant cells
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FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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Preparation of peptides or proteins
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having a known sequence of two or more amino acids, e.g. glutathione
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FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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Preparation of compounds containing saccharide radicals
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Preparation of nitrogen-containing carbohydrates
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N-glycosides
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Nucleotides
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Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Applicants:
ZYMERGEN INC. [US/US]; 5980 Horton Street Suite 105 Emeryville, California 94608, US
Inventors:
MANCHESTER, Shawn; US
NECKELMANN, Alexander; US
Agent:
HOLLY, David C.; US
MCEVOY, Michael; US
VEITENHEIMER, Erich E.; US
Priority Data:
62/356,93430.06.2016US
Title (EN) METHODS FOR GENERATING A BACTERIAL HEMOGLOBIN LIBRARY AND USES THEREOF
(FR) PROCÉDÉS DE GÉNÉRATION D'UNE BIBLIOTHÈQUE D'HÉMOGLOBINES BACTÉRIENNES ET UTILISATIONS ASSOCIÉES
Abstract:
(EN) The present disclosure describes methods for generating microbial strains expressing a heterologous bacterial hemoglobin gene that produce biomolecules of interest. In aspects, the disclosure provides novel bacterial strains, which express a heterologous bacterial hemoglobin gene whose expression is controlled by a native Corynebacterium glutamicum promoter or a mutant promoter derived therefrom. Also provided herein are methods for producing a library of bacterial hemoglobin genes using a promoter ladder comprising a plurality of promoters derived from Corynebacterium glutamicum.
(FR) La présente invention concerne des procédés de génération de souches microbiennes exprimant un gène d'hémoglobine bactérienne hétérologue produisant des biomolécules dignes d'intérêt. Selon certains aspects, l'invention concerne de nouvelles souches bactériennes qui expriment un gène d'hémoglobine bactérienne hétérologue dont l'expression est régulée par un promoteur natif de Corynebacterium glutamicum ou un promoteur mutant dérivé de celui-ci. L'invention concerne également des procédés de production d'une bibliothèque de gènes d'hémoglobines bactériennes à l'aide d'une gamme de promoteurs comprenant une pluralité de promoteurs dérivés de Corynebacterium glutamicum.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (EPO) (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)