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1. (WO2018005655) METHODS FOR GENERATING A BACTERIAL HEMOGLOBIN LIBRARY AND USES THEREOF

Pub. No.:    WO/2018/005655    International Application No.:    PCT/US2017/039772
Publication Date: Fri Jan 05 00:59:59 CET 2018 International Filing Date: Thu Jun 29 01:59:59 CEST 2017
IPC: C12N 15/09
C12N 15/67
C12N 15/52
C12N 15/70
C12N 15/10
C12N 15/77
C12N 15/82
C12P 21/02
C12P 19/34
Applicants: ZYMERGEN INC.
Inventors: MANCHESTER, Shawn
NECKELMANN, Alexander
Title: METHODS FOR GENERATING A BACTERIAL HEMOGLOBIN LIBRARY AND USES THEREOF
Abstract:
The present disclosure describes methods for generating microbial strains expressing a heterologous bacterial hemoglobin gene that produce biomolecules of interest. In aspects, the disclosure provides novel bacterial strains, which express a heterologous bacterial hemoglobin gene whose expression is controlled by a native Corynebacterium glutamicum promoter or a mutant promoter derived therefrom. Also provided herein are methods for producing a library of bacterial hemoglobin genes using a promoter ladder comprising a plurality of promoters derived from Corynebacterium glutamicum.