WIPO logo
Mobile | Deutsch | Español | Français | 日本語 | 한국어 | Português | Русский | 中文 | العربية |
PATENTSCOPE

Search International and National Patent Collections
World Intellectual Property Organization
Search
 
Browse
 
Translate
 
Options
 
News
 
Login
 
Help
 
Machine translation
1. (WO2018004067) ADIPONECTIN OVEREXPRESSION CELL LINE
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.:    WO/2018/004067    International Application No.:    PCT/KR2016/010584
Publication Date: 04.01.2018 International Filing Date: 22.09.2016
IPC:
C12N 15/85 (2006.01), C07K 14/575 (2006.01), C12P 21/00 (2006.01)
Applicants: INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY [KR/KR]; (Yongbong-dong) 77, Yongbong-ro, Buk-gu, Gwangju 61186 (KR)
Inventors: KIM, Seong Jin; (KR).
IM, Myung; (KR).
SOHN, Kyung Cheol; (KR).
JUNG, Yu Ra; (KR).
KIM, Chang Deok; (KR).
LEE, Jeung Hoon; (KR)
Agent: DOOHO IP LAW FIRM; (Seocho-dong, Daehyun Blue Tower) 7th Floor, 1, Gangnam-daero 51-gil, Seocho-gu, Seoul 06628 (KR)
Priority Data:
10-2016-0083513 01.07.2016 KR
Title (EN) ADIPONECTIN OVEREXPRESSION CELL LINE
(FR) LIGNÉE CELLULAIRE DE SUREXPRESSION D'ADIPONECTINE
(KO) 아디포넥틴 과발현 세포주
Abstract: front page image
(EN)The present invention relates to an adiponectin production method comprising the steps of: (a) producing an adiponectin expression vector by inserting adiponectin cDNA into a pcDNA 3.1 vector; (b) obtaining a cell line by transfecting the adiponectin expression vector into a eukaryotic cell; and (c) expressing the adiponectin in the cell line, and thus, through the adiponectin production method, post-translationally modified adiponectin may be overexpressed, polymer composite-type adiponectin may be produced at an affordable cost, the adiponectin may be used for effectively alleviating symptoms such as obesity, hyperglycemia, hyperinsulinism and cardiovascular diseases, and may be used for effectively alleviating skin diseases such as wounds, fibrosis, skin wrinkles and moisturization by promoting the expression of filaggrin, hyaluronic acid and an extracellular matrix, and polymer composite adiponectin may be produced at an affordable cost.
(FR)La présente invention concerne un procédé de production d'adiponectine comprenant les étapes consistant à : (a) produire un vecteur d'expression d'adiponectine par insertion d'ADNc d'adiponectine dans un vecteur de pcDNA3.1; (b) obtenir une lignée cellulaire par transfection du vecteur d'expression d'adiponectine dans une cellule eucaryote; et (c) exprimer l'adiponectine dans la lignée cellulaire et, par conséquent, par l'intermédiaire du procédé de production d'adiponectine, l'adiponectine modifiée de manière post-traductionnelle peut être surexprimée, l'adiponectine de type composite polymère peut être produite à un coût abordable, l'adiponectine peut être utilisée pour soulager efficacement des symptômes tels que l'obésité, l'hyperglycémie, l'hyperinsulinémie et les maladies cardiovasculaires et peut être utilisée pour soulager efficacement des maladies de la peau telles que les plaies, la fibrose, les rides et l'hydratation de la peau par le fait de favoriser l'expression de la filaggrine, de l'acide hyaluronique et d'une matrice extracellulaire et l'adiponectine composite polymère peut être produite à un coût abordable.
(KO)본 발명은 아디포넥틴을 생산하는 방법에 관한 것으로서, (a) 아디포넥틴 cDNA를 pcDNA 3.1 벡터에 삽입하여 아디포넥틴 발현 벡터를 제조하는 단계; (b) 상기 아디포넥틴 발현 벡터를 진핵 세포에 형질감염 (transfection)시켜 세포주를 수득하는 단계; 및 (c) 상기 세포주에서 아디포넥틴을 발현시키는 단계를 포함함으로써, 번역 후 변형된 아디포넥틴을 과발현할 수 있고, 고분자 복합체 형태의 아디포넥틴을 저렴한 가격으로 생산할 수 있으며, 비만, 고혈당, 고인슐린, 심혈관 질환 등의 증상을 효과적으로 완화시키는데 사용될 수 있으며 필라그린 (filaggrin), 하이루론산 (hyaluronicacid) 및 세포외기질 등의 발현을 촉진함으로써 상처, 섬유화, 피부주름, 보습 등의 피부 질환도 효과적으로 완화시키는데도 사용될 수 있으며, 고분자 복합체 아디포넥틴을 저렴한 가격으로 생산할 수 있는, 아디포넥틴을 생산하는 방법에 관한 것이다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: Korean (KO)
Filing Language: Korean (KO)