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1. (WO2018001304) RNA REVERSE TRANSCRIPTION AMPLIFICATION METHOD

Pub. No.:    WO/2018/001304    International Application No.:    PCT/CN2017/090759
Publication Date: Fri Jan 05 00:59:59 CET 2018 International Filing Date: Fri Jun 30 01:59:59 CEST 2017
IPC: C12Q 1/68
Applicants: XIAMEN UNIVERSITY
厦门大学
Inventors: ZHANG, Shiyin
张师音
GE, Shengxiang
葛胜祥
WANG, Jin
王进
ZHANG, Jun
张军
XIA, Ningshao
夏宁邵
Title: RNA REVERSE TRANSCRIPTION AMPLIFICATION METHOD
Abstract:
A ribonucleic acid (RNA) reverse transcription amplification method, comprising the steps of RNA reverse transcription into cDNA and immediate amplification on cDNA, characterized in that the process of RNA reverse transcription into cDNA was completed during the process that a cDNA amplification reaction system is heated to reach the reaction condition for cDNA amplification. The RNA reverse transcription amplification method can combine the reaction condition for RNA reverse transcription into cDNA with the reaction condition for cDNA amplification, thereby significantly shorten the time required for RNA reverse transcription amplification. And in the whole process of RNA reverse transcription amplification, there is no need to change the instrument temperature, thereby it is possible to achieve detection at any time.