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|1. (WO2018001241) METHOD FOR CONSTRUCTING PD-1 GENE-MODIFIED HUMANIZED ANIMAL MODEL AND USE THEREOF|
|Applicants:||BEIJING BIOCYTOGEN CO., LTD.
|Title:||METHOD FOR CONSTRUCTING PD-1 GENE-MODIFIED HUMANIZED ANIMAL MODEL AND USE THEREOF|
Provided is a method for preparing a PD-1 gene-modified humanized animal model. The method utilizes the CRIPSR/Cas9 technique to replace partial fragments of a mouse PD-1 gene with fragments of a human PD-1 gene using homologous recombination by constructing a target vector, and thereby preparing a gene-modified humanized mouse. This mouse can normally express a PD-1 protein containing the functional domain of the human PD-1 protein, and can be used as an animal model for mechanism research regarding PD-1, PD-L1 and other signals, a regulator screening, and a toxicological study; and has an important and high application value in the study of the function of the PD-1 gene, and in the development of new drugs.