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1. (WO2018001241) METHOD FOR CONSTRUCTING PD-1 GENE-MODIFIED HUMANIZED ANIMAL MODEL AND USE THEREOF

Pub. No.:    WO/2018/001241    International Application No.:    PCT/CN2017/090320
Publication Date: Fri Jan 05 00:59:59 CET 2018 International Filing Date: Wed Jun 28 01:59:59 CEST 2017
IPC: C07K 16/28
C12N 15/85
Applicants: BEIJING BIOCYTOGEN CO., LTD.
北京百奥赛图基因生物技术有限公司
Inventors: SHEN, Yuelei
沈月雷
BAI, Yang
白阳
HUANG, Rui
黄蕤
ZHOU, Xiaofei
周小飞
HU, Yuting
胡玉婷
GUO, Yanan
郭雅南
DU, Jichao
杜吉超
Title: METHOD FOR CONSTRUCTING PD-1 GENE-MODIFIED HUMANIZED ANIMAL MODEL AND USE THEREOF
Abstract:
Provided is a method for preparing a PD-1 gene-modified humanized animal model. The method utilizes the CRIPSR/Cas9 technique to replace partial fragments of a mouse PD-1 gene with fragments of a human PD-1 gene using homologous recombination by constructing a target vector, and thereby preparing a gene-modified humanized mouse. This mouse can normally express a PD-1 protein containing the functional domain of the human PD-1 protein, and can be used as an animal model for mechanism research regarding PD-1, PD-L1 and other signals, a regulator screening, and a toxicological study; and has an important and high application value in the study of the function of the PD-1 gene, and in the development of new drugs.