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1. (WO2017205868) CAMERA-BASED SINGLE MOLECULE READER

Pub. No.:    WO/2017/205868    International Application No.:    PCT/US2017/034988
Publication Date: Fri Dec 01 00:59:59 CET 2017 International Filing Date: Wed May 31 01:59:59 CEST 2017
IPC: H04N 5/217
G01N 21/63
G06T 7/136
Applicants: SINGULEX, INC.
Inventors: LIVINGSTON, Richard
ONORATO, Robert
WANG, Danni
GILBERT, Michele
Title: CAMERA-BASED SINGLE MOLECULE READER
Abstract:
A detector captures an image in response to one or more optical elements directing a first electromagnetic radiation to a binding surface. The image is defined by a plurality of pixels. Each pixel provides a signal. The signals provided by a subset of the pixels results at least in part from a second electromagnetic radiation emitted by target molecules bound to the binding surface, in response to the first electromagnetic radiation. The subset of pixels indicates a presence of the target molecules in corresponding areas of the binding surface. A processor determines a noise signal threshold based on an average background noise signal for the plurality of pixels. The processor identifies the subset of pixels indicating the presence of the target molecules by identifying the pixels having a signal greater than the noise signal threshold. The processor determines a count of the target molecules based on the subset of pixels.