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1. (WO2017205531) METHODS FOR VISUALIZATION AND QUANTIFICATION OF FIBER-LIKE STRUCTURES

Pub. No.:    WO/2017/205531    International Application No.:    PCT/US2017/034314
Publication Date: Fri Dec 01 00:59:59 CET 2017 International Filing Date: Thu May 25 01:59:59 CEST 2017
IPC: G01N 33/48
G01N 33/49
G01N 33/92
G02B 21/06
Applicants: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
Inventors: DEISSEROTH, Karl A.
YE, Li
MCNAB, Jennifer
TIAN, Qiyuan
Title: METHODS FOR VISUALIZATION AND QUANTIFICATION OF FIBER-LIKE STRUCTURES
Abstract:
Aspects of the present disclosure include a method for visualizing a fiber-like structure in a biological specimen, the method comprising: clearing the biological specimen comprising a fiber-like structure, wherein the fiber-like structure is detectably labeled; illuminating the cleared biological specimen with two light sheets from a first side and a second side to produce an image volume, wherein the second side is opposite to the first side and wherein the image volume comprises a representation of the fiber-like structure; defining a plurality of voxels within the representation of the fiber¬ like structure; processing each of the plurality of voxels to estimate a plurality of principal fiber-like structure orientations; and defining a starting point on the representation of the fiber-like structure and propagating a plurality of streamlines from the starting point, according to the plurality of principal fiber-like structure orientations, to visualize the fiber-like structure.