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1. (WO2017204572) METHOD FOR PREPARING LIBRARY FOR HIGHLY PARALLEL SEQUENCING BY USING MOLECULAR BARCODING, AND USE THEREOF
Latest bibliographic data on file with the International Bureau    Submit observation

Pub. No.:    WO/2017/204572    International Application No.:    PCT/KR2017/005455
Publication Date: 30.11.2017 International Filing Date: 25.05.2017
IPC:
C12Q 1/68 (2006.01), G06F 19/22 (2011.01)
Applicants: CELEMICS, INC. [KR/KR]; 1902, 1903, 1904, 1905-Na ho, 19th floor, A-dong, 131, Gasan digital 1-ro Geumcheon-gu Seoul 08506 (KR)
Inventors: KIM, Hyo Ki; (KR).
HAN, Hyo Jun; (KR).
SEO, Sung Hyun; (KR).
JANG, Hoon; (KR)
Agent: KWAK, Hyun Kyu; (KR).
LEE, Sung Ha; (KR)
Priority Data:
10-2016-0063919 25.05.2016 KR
Title (EN) METHOD FOR PREPARING LIBRARY FOR HIGHLY PARALLEL SEQUENCING BY USING MOLECULAR BARCODING, AND USE THEREOF
(FR) PROCÉDÉ DE PRÉPARATION DE BIBLIOTHÈQUE DESTINÉ À UN SÉQUENÇAGE HAUTEMENT PARALLÈLE À L'AIDE DU CODAGE À BARRES MOLÉCULAIRE ET SON UTILISATION
(KO) 분자 바코딩을 이용한 초병렬 시퀀싱을 위한 라이브러리 제조방법 및 그의 용도
Abstract: front page image
(EN)Provided is a method for preparing a library for highly parallel sequencing, comprising the steps of: providing two or more double stranded nucleic acid molecules; attaching adaptors to both ends of each of the nucleic acid molecules; providing a primer pair for amplifying each of the nucleic acid molecules, wherein each of the primers constituting the primer pair comprises i) a 3'-end region comprising a nucleotide sequence complementary to the adaptor, ii) a 5'-end region comprising a universal primer sequence for a highly parallel sequencing, and iii) an index sequence region positioned between the 3'- and 5'-end regions, and an index sequence of one of the primer pair is a unique molecular sequence unique to each of the nucleic acid molecules and the index sequence of the other primer is a sample indication sequence for indicating a sample from which the nucleic acid molecule is derived; and performing an amplification reaction by using the primer pair, so as to produce an amplification product of each of the nucleic acid molecules comprising the unique molecular sequence and the sample indication sequence.
(FR)La présente invention concerne un procédé de préparation d'une bibliothèque pour un séquençage hautement parallèle, comprenant les étapes suivantes : la fourniture d’au moins deux molécules d'acide nucléique double brin ; la fixation d’adaptateurs aux deux extrémités de chacune des molécules d'acide nucléique ; la fourniture de paires d'amorces pour amplifier chacune des molécules d'acide nucléique, chaque amorce constituant la paire d'amorces comprenant i) une région extrémité 3' comprenant une séquence nucléotidique complémentaire de l'adaptateur, ii) une région extrémité 5' comprenant une séquence d'amorce universelle pour un séquençage hautement parallèle, et iii) une région séquence d'index positionnée entre les régions extrémité 3' et 5', et une séquence d'index de l'une des amorces de la paire d'amorces étant une séquence moléculaire unique, propre à chacune des molécules d'acide nucléique et la séquence d'index de l'autre amorce étant une séquence d'indication d'échantillon pour indiquer l'échantillon à partir duquel la molécule d'acide nucléique est dérivée ; et la réaction d'amplification au moyen des paires d'amorces, de manière à produire des produits d'amplification de chacune des molécules d'acide nucléique comprenant la séquence moléculaire unique et la séquence d'indication d'échantillon.
(KO)2 이상의 이중가닥 핵산분자를 제공하는 단계; 상기 각 핵산분자의 양 말단에 어댑터를 부착하는 단계; 상기 각 핵산분자를 증폭하기 위한 프라이머 쌍을 제공하는 단계로서, 상기 프라이머 쌍을 이루는 프라이머 각각은 i) 상기 어댑터에 상보적인 뉴클레오티드 서열을 갖는 3'-말단 부위; ii) 초병렬 시퀀싱을 위한 공통 프라이머 서열을 갖는 5'-말단 부위; 및 iii) 상기 3'-말단 부위와 5'-말단 부위 사이에 위치하는 인덱스 서열 부위를 포함하고, 상기 프라이머 쌍 중 하나의 인덱스 서열은 각 핵산분자에 대해 고유한 분자 고유 서열이고 나머지 하나의 인덱스 서열은 핵산분자가 유래된 시료를 표시하는 시료 표시 서열인 것인 단계; 및 상기 프라이머 쌍을 이용하여 증폭반응을 수행하여, 분자 고유 서열 및 시료 표시 서열을 포함하는 상기 각 핵산분자의 증폭산물을 생성하는 단계를 포함하는, 초병렬 시퀀싱을 위한 라이브러리를 제조하는 방법이 제공된다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, RU, TJ, TM)
European Patent Office (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG).
Publication Language: Korean (KO)
Filing Language: Korean (KO)