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1. (WO2017201105) METHOD FOR PURIFICATION AND ACTIVATION OF BOTULINUM NEUROTOXIN

Pub. No.:    WO/2017/201105    International Application No.:    PCT/US2017/032985
Publication Date: Fri Nov 24 00:59:59 CET 2017 International Filing Date: Wed May 17 01:59:59 CEST 2017
IPC: C07K 14/33
C07K 1/22
C12N 9/52
Applicants: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Inventors: DONG, Min
BARKHO, Sulyman
TAO, Liang
Title: METHOD FOR PURIFICATION AND ACTIVATION OF BOTULINUM NEUROTOXIN
Abstract:
Disclosed herein are methods for the isolation and purification of a botulinum neurotoxin (BoNT) protein, or a polypeptide comprising a receptor binding domain of BoNT, from a solution. The method comprises contacting the solution containing the protein or polypeptide to a matrix which has attached thereto a non-toxic non-hemagglutinin (NTNHA) under conditions appropriate for binding, washing the matrix to thereby remove unbound materials, and eluting the protein or polypeptide with a solution that dissociates the bound protein from the NTNHA. Conditions appropriate for binding are a pH of less than 7.5 (e.g, 6). Conditions appropriate for dissociation are a pH greater than or equal to 7.5 (e.g., 8). Compositions specific to the methods are also disclosed.